Discovery of Tumor Markers for Gastric Cancer by Proteomics

被引：33
作者：
Wu, Jeng-Yih [1 ,2 ,3 ]
Cheng, Chun-Chia [4 ]
Wang, Jaw-Yuan [5 ]
Wu, Deng-Chyang [2 ,3 ,6 ]
Hsieh, Jan-Sing [5 ]
Lee, Shui-Cheng [2 ]
Wang, Wen-Ming [1 ,2 ,3 ]
机构：
[1] Kaohsiung Municipal Tatung Hosp, Dept Internal Med, Kaohsiung, Taiwan
[2] Kaohsiung Med Univ Hosp, Div Gastroenterol, Dept Internal Med, Kaohsiung, Taiwan
[3] Kaohsiung Med Univ, Dept Med, Coll Med, Fac Med, Kaohsiung, Taiwan
[4] Atom Energy Council, Inst Nucl Energy Res, Executive Yuan, Taiwan
[5] Kaohsiung Med Univ Hosp, Dept Surg, Kaohsiung, Taiwan
[6] Kaohsiung Med Univ, Kaohsiung Municipal Hsiao Kang Hosp, Dept Internal Med, Kaohsiung, Taiwan
来源：
PLOS ONE | 2014年 / 9卷 / 01期
关键词：
REGULATED PROTEINS GRP78; S-TRANSFERASE-PI; HEPATOCELLULAR-CARCINOMA; EXPRESSION; GRP94; ALPHA-1-ANTITRYPSIN; OVEREXPRESSION; EPITHELIUM; DIAGNOSIS; SURVIVAL;
D O I：
10.1371/journal.pone.0084158
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Gastric cancer (GC) has a high rate of morbidity and mortality among various cancers worldwide. The development of noninvasive diagnostic methods or technologies for tracking the occurrence of GC is urgent, and searching reliable biomarkers is considered. This study intended to directly discover differential biomarkers from GC tissues by two-dimension-differential gel electrophoresis (2D-DIGE), and further validate protein expression by western blotting (WB) and immunohistochemistry (IHC). Pairs of GC tissues (gastric cancer tissues and the adjacent normal tissues) obtained from surgery was investigated for 2D-DIEG. Five proteins were confirmed by WB and IHC, including glucose-regulated protein 78 (GRP78), glutathione s-transferase pi (GSTpi), apolipoprotein AI (ApoAI), alpha-1 antitrypsin (A1AT) and gastrokine-1 (GKN-1). Among the results, GRP78, GSTpi and A1AT were significantly up-regulated and down-regulated respectively in gastric cancer patients. Moreover, GRP78 and ApoAI were correlated with A1AT for protein expressions. This study presumes these proteins could be candidates of reliable biomarkers for gastric cancer.
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页数：7
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