Optimization of Subcritical Water Extraction (SWE) of Lipid and Eicosapentaenoic Acid (EPA) from Nannochloropsis gaditana

被引:17
作者
Ho, Bernard Chon Han [1 ]
Kamal, Siti Mazlina Mustapa [2 ]
Danquah, Michael K. [3 ]
Harun, Razif [1 ]
机构
[1] Univ Putra Malaysia, Dept Chem & Environm Engn, Fac Engn, Serdang 43400, Malaysia
[2] Univ Putra Malaysia, Dept Proc & Food Engn, Fac Engn, Serdang 43400, Malaysia
[3] Curtin Univ, Dept Chem & Petr Engn, Sarawak, Malaysia
关键词
ULTRASOUND-ASSISTED EXTRACTION; HYDROTHERMAL LIQUEFACTION; FATTY-ACIDS; BIOMASS; MICROALGAE; BIODIESEL; OMEGA-3-FATTY-ACIDS; METAANALYSIS; COMPONENTS; FISH;
D O I
10.1155/2018/8273581
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Microalgae are a promising source of omega-3. The purpose of this study was to extract lipid with a relatively high content of eicosapentaenoic acid (EPA) from Nannochloropsis gaditana using subcritical water extraction (SWE). The effects of different temperatures (156.1-273.9 degrees C), extraction times (6.6-23.4 minutes), and biomass loadings (33-117g algae/L) on the extraction yield were studied. From the optimization study using central composite design (CCD), quadratic models generated for lipid yield and EPA composition were considered to be significant models (p < 0.05). The predictive equations were also formed for lipid yield and EPA composition. The predicted optimum lipid yield and EPA composition at 236.54 degrees C, 13.95 minutes, and 60.50g algae/L were 18.278wt% of total biomass and 14.036wt% of total fatty acid methyl ester (FAME), respectively.
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页数:11
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