Anthrax toxin receptor 2a controls mitotic spindle positioning

被引：39

作者：

Castanon, I. ^{[2
,3
]}

Abrami, L. ^{[1
]}

Holtzer, L. ^{[2
,3
]}

Heisenberg, C. P. ^{[4
]}

van der Goot, F. G. ^{[1
]}

Gonzalez-Gaitan, M. ^{[2
,3
]}

机构：

[1] Ecole Polytech Fed Lausanne, Global Hlth Inst, CH-1015 Lausanne, Switzerland

[2] Dept Biochem, CH-1211 Geneva 4, Switzerland

[3] Dept Mol Biol, CH-1211 Geneva 4, Switzerland

[4] IST Austria, A-3400 Klosterneuburg, Austria

来源：

NATURE CELL BIOLOGY | 2013年 / 15卷 / 01期

关键词：

CELL-DIVISION ORIENTATION; ZEBRAFISH GASTRULATION; RHO GTPASES; F-ACTIN; EXTENSION MOVEMENTS; IN-VIVO; POLARITY; PROTEIN; EXPRESSION; MECHANISM;

D O I：

10.1038/ncb2632

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Oriented mitosis is essential during tissue morphogenesis. The Wnt/planar cell polarity (Wnt/PCP) pathway orients mitosis in a number of developmental systems, including dorsal epiblast cell divisions along the animal-vegetal (A-V) axis during zebrafish gastrulation. How Wnt signalling orients the mitotic plane is, however, unknown. Here we show that, in dorsal epiblast cells, anthrax toxin receptor 2a (Antxr2a) accumulates in a polarized cortical cap, which is aligned with the embryonic A-V axis and forecasts the division plane. Filamentous actin (F-actin) also forms an A-V polarized cap, which depends on Wnt/PCP and its effectors RhoA and Rock2. Antxr2a is recruited to the cap by interacting with actin. Antxr2a also interacts with RhoA and together they activate the diaphanous-related formin zDia2. Mechanistically, Antxr2a functions as a Wnt-dependent polarized determinant, which, through the action of RhoA and zDia2, exerts torque on the spindle to align it with the A-V axis.

引用

页码：28 / U71

页数：23

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