Enzyme association with lipidic Langmuir-Blodgett films: Interests and applications in nanobioscience

被引:145
作者
Girard-Egrot, AP [1 ]
Godoy, S [1 ]
Blum, LJ [1 ]
机构
[1] Univ Lyon 1, CNRS, UMR 5013, EMB2,Lab Genie Enzymat & Biomol, F-69622 Villeurbanne, France
关键词
Langmuir-Blodgett films; biomimetic nanostructure; protein orientation; biosensing devices; enzyme kinetic behaviour;
D O I
10.1016/j.cis.2005.04.006
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
This review presents the recent advances in the achievement of organized proteo-lipidic nanostructures based on Langmuir-Blodgett technology and their potential applications in the nanobioscience area. By using the self-assembled properties of amphiphilic biomolecules at the air-water interface, the Langmuir-Blodgett (LB) technique offers the possibility to prepare ultrathin layers suitable for biomolecule immobilization at the molecular level. This review will provide a general overview of the enzyme association with preformed Langmuir-Blodgett films in connection with their potential applications in biosensing device developments, and then introduce the design of a new functionalised biomimetic nanostructure with oriented recognition site. The potential applications of such an organized proteo-lipidic nanostructure for biocatalysis investigations of an immobilised enzyme in a biomimetic situation and for the development of bioelectronic devices are finally discussed. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:205 / 225
页数:21
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