Comparative Analysis of Benzoxazinoid Biosynthesis in Monocots and Dicots: Independent Recruitment of Stabilization and Activation Functions

被引：55

作者：

Dick, Regina ^{[1
]}

Rattei, Thomas ^{[2
]}

Haslbeck, Martin ^{[3
]}

Schwab, Wilfried

Gierl, Alfons ^{[1
]}

Frey, Monika ^{[1
]}

机构：

[1] Tech Univ Munich, Lehrstuhl Genet, Wissensch Zentrum Weihenstephan, D-85354 Freising Weihenstephan, Germany

[2] Tech Univ Munich, Dept Genome Oriented Bioinformat, Wissensch Zentrum Weihenstephan, D-85350 Freising Weihenstephan, Germany

[3] Tech Univ Munich, Lehrstuhl Biotechnol, Dept Chem, D-85747 Garching, Germany

来源：

PLANT CELL | 2012年 / 24卷 / 03期

关键词：

BETA-GLUCOSIDASE ACTIVITY; HYDROXAMIC ACID CONTENT; MOLECULAR CHARACTERIZATION; LOTUS-JAPONICUS; SECALE-CEREALE; BIOCHEMICAL-CHARACTERIZATION; SUBCELLULAR-LOCALIZATION; CHROMOSOMAL LOCALIZATION; SUBSTRATE-SPECIFICITY; CYANOGENIC GLYCOSIDES;

D O I：

10.1105/tpc.112.096461

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Benzoxazinoids represent preformed protective and allelophatic compounds that are found in a multitude of species of the family Poaceae (Gramineae) and occur sporadically in single species of phylogenetically unrelated dicots. Stabilization by glucosylation and activation by hydrolysis is essential for the function of these plant defense compounds. We isolated and functionally characterized from the dicot larkspur (Consolida orientalis) the benzoxazinoid-specific UDP-glucosyltransferase and beta-glucosidase that catalyze the enzymatic functions required to avoid autotoxicity and allow activation upon challenge by herbivore and pathogen attack. A phylogenetic comparison of these enzymes with their counterparts in the grasses indicates convergent evolution by repeated recruitment from homologous but not orthologous genes. The data reveal a great evolutionary flexibility in recruitment of these essential functions of secondary plant metabolism.

引用

页码：915 / 928

页数：14

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