Use of spectrophotometric reading for in vitro antifungal susceptibility testing of Aspergillus spp.

被引：14

作者：

Dannaoui, E ^{[1
]}

Persat, F ^{[1
]}

Monier, MF ^{[1
]}

Borel, E ^{[1
]}

Piens, MA ^{[1
]}

Picot, S ^{[1
]}

机构：

[1] Univ Lyon 1, Lab Parasitol Mycol Med & Pathol Exot, F-69373 Lyon, France

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1999年 / 45卷 / 10期

关键词：

antifungal; susceptibility testing; Aspergillus; spectrophotometric reading;

D O I：

10.1139/cjm-45-10-871

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A comparative study of visual and spectrophotometric MIC endpoint determinations for antifungal susceptibility testing of Aspergillus species was performed. A broth microdilution method adapted from the National Committee for Clinical Laboratory Standards (NCCLS) was used for susceptibility testing of 180 clinical isolates of Aspergillus species against amphotericin B and itraconazole. MICs were determined visually and spectrophotometrically at 490 nm after 24, 48, and 72h of incubation, and MIC pairs were compared. The agreement between the two methods was 99% for amphotericin B and ranged from 95 to 98% for itraconazole. It is concluded that spectrophotometric MIC endpoint determination is a valuable alternative to the Visual reference method for susceptibility testing of Aspergillus species.

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收藏

页码：871 / 874

页数：4

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