Display of Clostridium cellulovorans Xylose Isomerase on the Cell Surface of Saccharomyces cerevisiae and its Direct Application to Xylose Fermentation

被引：29

作者：

Ota, Miki ^{[1
]}

Sakuragi, Hiroshi ^{[1
]}

Morisaka, Hironobu ^{[1
]}

Kuroda, Kouichi ^{[1
]}

Miyake, Hideo ^{[2
]}

Tamaru, Yutaka ^{[2
]}

Ueda, Mitsuyoshi ^{[1
]}

机构：

[1] Kyoto Univ, Div Appl Life Sci, Grad Sch Agr, Sakyo Ku, Kyoto 6068502, Japan

[2] Mie Univ, Grad Sch Bioresources, Dept Life Sci, Tsu, Mie 5148507, Japan

来源：

BIOTECHNOLOGY PROGRESS | 2013年 / 29卷 / 02期

关键词：

xylose isomerase; Clostridium cellulovorans; Saccharomyces cerevisiae; cell surface engineering; ETHANOLIC FERMENTATION; FUNCTIONAL EXPRESSION; YEAST; GENE; CONSTRUCTION; PROTEINS; CLONING;

D O I：

10.1002/btpr.1700

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Xylose isomerase (XI) is a key enzyme in the conversion of d-xylose, which is a major component of lignocellulosic biomass, to d-xylulose. Genomic analysis of the bacterium Clostridium cellulovorans revealed the presence of XI-related genes. In this study, XI derived from C. cellulovorans was produced and displayed using the yeast cell-surface display system, and the xylose assimilation and fermentation properties of this XI-displaying yeast were examined. XI-displaying yeast grew well in medium containing xylose as the sole carbon source and directly produced ethanol from xylose under anaerobic conditions. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29: 346351, 2013

引用

页码：346 / 351

页数：6

共 31 条

[1] THE FERMENTATION OF XYLOSE - AN ANALYSIS OF THE EXPRESSION OF BACILLUS AND ACTINOPLANES XYLOSE ISOMERASE GENES IN YEAST [J].

AMORE, R ;

WILHELM, M ;

HOLLENBERG, CP .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1989, 30 (04) :351-357

[2] Functional Expression of a Bacterial Xylose Isomerase in Saccharomyces cerevisiae [J].

Brat, Dawid ;

Boles, Eckhard ;

Wiedemann, Beate .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (08) :2304-2311

[3]

CARRELL HL, 1984, J BIOL CHEM, V259, P3230

[4] CLONING THE YEAST XYLULOKINASE GENE FOR THE IMPROVEMENT OF XYLOSE FERMENTATION [J].

CHANG, SF ;

HO, NWY .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1988, 17 :313-318

[5] D-XYLULOSE FERMENTATION TO ETHANOL BY SACCHAROMYCES-CEREVISIAE [J].

CHIANG, LC ;

GONG, CS ;

CHEN, LF ;

TSAO, GT .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1981, 42 (02) :284-289

[6] CHARACTERIZATION AND COMPARISON OF CLOSTRIDIUM-CELLULOVORANS ENDOGLUCANASES-XYLANASES ENGB AND ENGD HYPEREXPRESSED IN ESCHERICHIA-COLI [J].

FOONG, FCF ;

DOI, RH .

JOURNAL OF BACTERIOLOGY, 1992, 174 (04) :1403-1409

[7] Synergistic saccharification, and direct fermentation to ethanol, of amorphous cellulose by use of an engineered yeast strain codisplaying three types of cellulolytic enzyme [J].

Fujita, Y ;

Ito, J ;

Ueda, M ;

Fukuda, H ;

Kondo, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (02) :1207-1212

[8]

Hess JM, 1998, APPL ENVIRON MICROB, V64, P2357

[9] TRANSFORMATION OF INTACT YEAST-CELLS TREATED WITH ALKALI CATIONS [J].

ITO, H ;

FUKUDA, Y ;

MURATA, K ;

KIMURA, A .

JOURNAL OF BACTERIOLOGY, 1983, 153 (01) :163-168

[10] Alteration of substrate specificity of rat neurolysin from matrix metalloproteinase-2/9-type to-3-type specificity by comprehensive mutation [J].

Kadonosono, Tetsuya ;

Kato-Murai, Michiko ;

Ueda, Mitsuyoshi .

PROTEIN ENGINEERING DESIGN & SELECTION, 2008, 21 (08) :507-513

← 1 2 3 4 →