Display of Clostridium cellulovorans Xylose Isomerase on the Cell Surface of Saccharomyces cerevisiae and its Direct Application to Xylose Fermentation

被引：28
|
作者：
Ota, Miki [1 ]
Sakuragi, Hiroshi [1 ]
Morisaka, Hironobu [1 ]
Kuroda, Kouichi [1 ]
Miyake, Hideo [2 ]
Tamaru, Yutaka [2 ]
Ueda, Mitsuyoshi [1 ]
机构：
[1] Kyoto Univ, Div Appl Life Sci, Grad Sch Agr, Sakyo Ku, Kyoto 6068502, Japan
[2] Mie Univ, Grad Sch Bioresources, Dept Life Sci, Tsu, Mie 5148507, Japan
来源：
BIOTECHNOLOGY PROGRESS | 2013年 / 29卷 / 02期
关键词：
xylose isomerase; Clostridium cellulovorans; Saccharomyces cerevisiae; cell surface engineering; ETHANOLIC FERMENTATION; FUNCTIONAL EXPRESSION; YEAST; GENE; CONSTRUCTION; PROTEINS; CLONING;
D O I：
10.1002/btpr.1700
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Xylose isomerase (XI) is a key enzyme in the conversion of d-xylose, which is a major component of lignocellulosic biomass, to d-xylulose. Genomic analysis of the bacterium Clostridium cellulovorans revealed the presence of XI-related genes. In this study, XI derived from C. cellulovorans was produced and displayed using the yeast cell-surface display system, and the xylose assimilation and fermentation properties of this XI-displaying yeast were examined. XI-displaying yeast grew well in medium containing xylose as the sole carbon source and directly produced ethanol from xylose under anaerobic conditions. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29: 346351, 2013
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收藏
页码：346 / 351
页数：6
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