Gradient stationary phase optimized selectivity liquid chromatography with conventional columns

被引：24

作者：

Chen, Kai ^{[1
]}

Lynen, Frederic ^{[1
]}

Szucs, Roman ^{[2
]}

Hanna-Brown, Melissa ^{[2
]}

Sandra, Pat ^{[1
,3
]}

机构：

[1] Univ Ghent, Pfizer Analyt Res Ctr, B-9000 Ghent, Belgium

[2] Pfizer Global Res & Dev, Sandwich CT13 9NJ, Kent, England

[3] Res Inst Chromatog, B-8500 Kortrijk, Belgium

来源：

ANALYST | 2013年 / 138卷 / 10期

关键词：

LC;

D O I：

10.1039/c3an36797e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Stationary phase optimized selectivity liquid chromatography (SOSLC) is a promising technique to optimize the selectivity of a given separation. By combination of different stationary phases, SOSLC offers excellent possibilities for method development under both isocratic and gradient conditions. The so far available commercial SOSLC protocol utilizes dedicated column cartridges and corresponding cartridge holders to build up the combined column of different stationary phases. The present work is aimed at developing and extending the gradient SOSLC approach towards coupling conventional columns. Generic tubing was used to connect short commercially available LC columns. Fast and base-line separation of a mixture of 12 compounds containing phenones, benzoic acids and hydroxybenzoates under both isocratic and linear gradient conditions was selected to demonstrate the potential of SOSLC. The influence of the connecting tubing on the deviation of predictions is also discussed.

引用

页码：2914 / 2923

页数：10

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