Evaluation of four selective agars and two enrichment broths in screening for methicillin-resistant Staphylococcus aureus

被引:38
作者
Bocher, S. [1 ]
Smyth, R. [2 ]
Kahlmeter, G. [2 ]
Kerremans, J. [3 ]
Vos, M. C. [3 ]
Skov, R. [1 ]
机构
[1] Statens Serum Inst, Natl Ctr Antimicrobials & Infect Control, DK-2300 Copenhagen, Denmark
[2] Cent Hosp, Swedish External Reference Ctr Antimicrobial Susc, Vaxio, Sweden
[3] Erasmus Univ Ctr, Dept Med Microbiol & Infect Dis, Rotterdam, Netherlands
关键词
D O I
10.1128/JCM.00478-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To evaluate methicillin-resistant Staphylococcus aureus detection, we tested in vitro four selective agars and two enrichment broths apart and in combination. Tryptone soya broth with salt, aztreonam, and cefoxitin appeared to be the most sensitive medium. This broth was superior to a phenol red mannitol broth with aztreonam and ceftizoxime.
引用
收藏
页码:3136 / 3138
页数:3
相关论文
共 16 条
[1]   Does nasal cocolonization by methicillin-resistant coagulase-negative Staphylococci and methicillin-susceptible Staphylococcus aureus strains occur frequently enough to represent a risk of false-positive methicillin-resistant S. aureus determinations by molecular methods? [J].
Becker, K ;
Pagnier, I ;
Schuhen, B ;
Wenzelburger, F ;
Friedrich, AW ;
Kipp, F ;
Peters, G ;
von Eiff, C .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (01) :229-231
[2]   Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA) [J].
Brown, DFJ ;
Edwards, DI ;
Hawkey, PM ;
Morrison, D ;
Ridgway, GL ;
Towner, KJ ;
Wren, MWD .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2005, 56 (06) :1000-1018
[3]   Salt tolerance of methicillin-resistant and methicillin-susceptible Staphylococcus aureus [J].
Bruins, Marjan J. ;
Juffer, Petra ;
Wolfhagen, Maurice J. H. M. ;
Ruijs, Gijs J. H. M. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (02) :682-683
[4]   EXPRESSION OF METHICILLIN RESISTANCE IN HETEROGENEOUS STRAINS OF STAPHYLOCOCCUS-AUREUS [J].
HARTMAN, BJ ;
TOMASZ, A .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1986, 29 (01) :85-92
[5]   Salt tolerance of EMRSA-16 and its effect on the sensitivity of screening cultures [J].
Jones, EM ;
Bowker, KE ;
Cooke, R ;
Marshall, RJ ;
Reeves, DS ;
MacGowan, AP .
JOURNAL OF HOSPITAL INFECTION, 1997, 35 (01) :59-62
[6]   Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment:: Rapid identification system for mec, ccr, and major differences in junkyard regions [J].
Kondo, Yoko ;
Ito, Teruyo ;
Ma, Xiao Xue ;
Watanabe, Shinya ;
Kreiswirth, Barry N. ;
Etienne, Jerome ;
Hiramatsu, Keiichi .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007, 51 (01) :264-274
[7]   Detection and expression of methicillin/oxacillin resistance in multidrug-resistant and non-multidrug-resistant Staphylococcus aureus in Central Sydney, Australia [J].
Merlino, J ;
Watson, J ;
Rose, B ;
Beard-Pegler, M ;
Gottlieb, T ;
Bradbury, R ;
Harbour, C .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2002, 49 (05) :793-801
[8]  
Merlino J, 2000, J CLIN MICROBIOL, V38, P2378
[9]   Soft salt-mannitol agar-cloxacillin test:: A highly specific bedside screening test for detection of colonization with methicillin-resistant Staphylococcus aureus [J].
Mir, N ;
Sánchez, M ;
Baquero, F ;
López, B ;
Calderón, C ;
Cantón, R .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (04) :986-989
[10]   Evaluation of three chromogenic media (MRSA-ID, MRSA-Select and CHROMagar MRSA) and ORSAB for surveillance cultures of methicillin-resistant Staphylococcus aureus [J].
Nahimana, I. ;
Francioli, P. ;
Blanc, D. S. .
CLINICAL MICROBIOLOGY AND INFECTION, 2006, 12 (12) :1168-1174