Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway

被引：52

作者：

Lv, Runxiao ^{[1
]}

Du, Lili ^{[2
]}

Liu, Xueyong ^{[1
]}

Zhou, Fenghua ^{[1
]}

Zhang, Zhiqiang ^{[1
]}

Zhang, Lixin ^{[1
]}

机构：

[1] China Med Univ, Shengjing Hosp, Dept Rehabil Med, 36 Sanhao St, Shenyang 110004, Liaoning, Peoples R China

[2] China Med Univ, Coll Basic Med Sci, Dept Pathophysiol, Shenyang 110122, Liaoning, Peoples R China

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2019年 / 70卷

关键词：

Spinal cord injury; Polydatin; Microglia; NO; NLRP3; inflammasome; CENTRAL-NERVOUS-SYSTEM; NITRIC-OXIDE; ISCHEMIA/REPERFUSION INJURY; IMPROVES RECOVERY; OXIDATIVE STRESS; UP-REGULATION; RAT MODELS; ACTIVATION; APOPTOSIS; CELLS;

D O I：

10.1016/j.intimp.2019.02.006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Polydatin is a glucoside of resveratrol with lots of functional properties in the central nervous system, such as anti-edema, anti-oxidation and anti-inflammation. The purpose of this study was to evaluate the effects of polydatin on traumatic spinal cord injury (SCI) and explore the relative mechanisms. SCI models were established using the weight-drop method in rats, additionally, single polydatin administration (20, 40 mg/kg body weight) remarkably improved motor function of SCI rat, along with decreased nitric oxide (NO) generation and inflammatory factor (IL-1 beta, IL-6 and TNF-alpha) production in spinal cord tissues. Similar to the results of in vivo experiments, the inflammatory response was aggravated with the intervention of lipopolysaccharide (LPS) in BV2 microglia. However, polydatin treatment (1, 2 and 4 mu M) inhibited iNOS expression, decreased NLRP3 inflammasome activation, which subsequently relieved microglial inflammation. Above all, our data indicated that polydatin possessed neuroprotective effects in SCI rats, possibly by suppressing iNOS expression and NLRP3 inflammasome activation in microglia.

引用

页码：28 / 36

页数：9

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