Engineering G protein-coupled receptor expression in bacteria

被引:6
作者
Skretas, Georgios [1 ,4 ]
Georgiou, George [1 ,2 ,3 ,4 ]
机构
[1] Univ Texas Austin, Dept Chem Engn, Austin, TX 78712 USA
[2] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA
[3] Univ Texas Austin, Dept Mol Genet & Microbiol, Austin, TX 78712 USA
[4] Univ Texas Austin, Inst Cell & Mol Biol, Austin, TX 78712 USA
关键词
D O I
10.1073/pnas.0807741105
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
引用
收藏
页码:14747 / 14748
页数:2
相关论文
共 18 条
[1]   Constitutive activation of the neurotensin receptor 1 by mutation of Phe358 in Helix seven [J].
Barroso, S ;
Richard, F ;
Nicolas-Ethève, D ;
Kitabgi, P ;
Labbé-Jullié, C .
BRITISH JOURNAL OF PHARMACOLOGY, 2002, 135 (04) :997-1002
[2]   Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS) [J].
Chen, G ;
Hayhurst, A ;
Thomas, JG ;
Harvey, BR ;
Iverson, BL ;
Georgiou, G .
NATURE BIOTECHNOLOGY, 2001, 19 (06) :537-542
[3]   High-resolution crystal structure of an engineered human β2-adrenergic G protein-coupled receptor [J].
Cherezov, Vadim ;
Rosenbaum, Daniel M. ;
Hanson, Michael A. ;
Rasmussen, Soren G. F. ;
Thian, Foon Sun ;
Kobilka, Tong Sun ;
Choi, Hee-Jung ;
Kuhn, Peter ;
Weis, William I. ;
Kobilka, Brian K. ;
Stevens, Raymond C. .
SCIENCE, 2007, 318 (5854) :1258-1265
[4]   EXPRESSION OF A RAT NEUROTENSIN RECEPTOR IN ESCHERICHIA-COLI [J].
GRISSHAMMER, R ;
DUCKWORTH, R ;
HENDERSON, R .
BIOCHEMICAL JOURNAL, 1993, 295 :571-576
[5]   The 7TM G-protein-coupled receptor target family [J].
Jacoby, Edgar ;
Bouhelal, Rochdi ;
Gerspacher, Marc ;
Seuwen, Klaus .
CHEMMEDCHEM, 2006, 1 (08) :760-782
[6]   Protein design by directed evolution [J].
Jaeckel, Christian ;
Kast, Peter ;
Hilvert, Donald .
ANNUAL REVIEW OF BIOPHYSICS, 2008, 37 :153-173
[7]   New G-protein-coupled receptor crystal structures: insights and limitations [J].
Kobilka, Brian ;
Schertler, Gebhard F. X. .
TRENDS IN PHARMACOLOGICAL SCIENCES, 2008, 29 (02) :79-83
[8]   Co-evolving stability and conformational homogeneity of the human adenosine A2a receptor [J].
Magnani, Francesca ;
Shibata, Yoko ;
Serrano-Vega, Maria J. ;
Tate, Christopher G. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (31) :10744-10749
[9]   Engineering membrane protein overproduction in Escherichia coli [J].
Molina, Daniel Martinez ;
Cornvik, Tobias ;
Eshaghi, Said ;
Haeggstrom, Jesper Z. ;
Nordlund, Par ;
Sabet, Marina Ignatushchenko .
PROTEIN SCIENCE, 2008, 17 (04) :673-680
[10]   Direct proteolysis-based purification of an overexpressed hyperthermophile protein from Escherichia coli lysate:: a novel exploitation of the link between structural stability and proteolytic resistance [J].
Mukherjee, S ;
Guptasarma, P .
PROTEIN EXPRESSION AND PURIFICATION, 2005, 40 (01) :71-76