Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)

被引：4

作者：

Lai, Meng Yee ^{[1
]}

Bukhari, Fatma Diyana Mohd ^{[1
]}

Zulkefli, Nur Zulaikha ^{[1
]}

Ismail, Ilyiana ^{[2
]}

Mustapa, Nur Izati ^{[2
]}

Soh, Tuan Suhaila Tuan ^{[2
]}

Hassan, Afifah Haji ^{[2
]}

Peariasamy, Kalaiarasu M. ^{[3
]}

Lee, Yee Leng ^{[4
]}

Suppiah, Jeyanthi ^{[5
]}

Thayan, Ravindran ^{[5
]}

Isa, Mohd Khairi Mat ^{[6
]}

Wahid, Nur Zafirah Abdul ^{[6
]}

Lau, Yee Ling ^{[1
]}

机构：

[1] Univ Malaya, Fac Med, Dept Parasitol, Kuala Lumpur 50603, Malaysia

[2] Minist Hlth, Hosp Sungai Buloh, Dept Pathol, Kuala Lumpur, Malaysia

[3] Minist Hlth, Inst Clin Res, Natl Inst Hlth, Kuala Lumpur, Malaysia

[4] Minist Hlth, Clin Res Ctr, Hosp Sungai Buloh, Kuala Lumpur, Malaysia

[5] Minist Hlth, Infect Dis Res Ctr, Natl Inst Hlth, Virol Unit,Inst Med Res, Kuala Lumpur, Malaysia

[6] Selia Tek Holdings Sdn Bhd, Lot 18,Jalan Teknol 3-5, Kota Damansara 47810, Selangor, Malaysia

来源：

BMC INFECTIOUS DISEASES | 2022年 / 22卷 / 01期

关键词：

Diagnosis; RT-LAMP; SARS-CoV-2; Phenol red; pH sensitive indicator; VISUAL DETECTION;

D O I：

10.1186/s12879-022-07684-w

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background High cost of commercial RNA extraction kits limits the testing efficiency of SARS-CoV-2. Here, we developed a simple nucleic acid extraction method for the detection of SARS-CoV-2 directly from nasopharyngeal swab samples. Methods A pH sensitive dye was used as the end point detection method. The obvious colour changes between positive and negative reactions eliminates the need of other equipment. Results Clinical testing using 260 samples showed 92.7% sensitivity (95% CI 87.3-96.3%) and 93.6% specificity (95% CI 87.3-97.4%) of RT-LAMP. Conclusions The simple RNA extraction method minimizes the need for any extensive laboratory set-up. We suggest combining this simple nucleic acid extraction method and RT-LAMP technology as the point-of care diagnostic tool.

引用

页数：5

共 17 条

[1] A molecular test based on RT-LAMP for rapid, sensitive and inexpensive colorimetric detection of SARS-CoV-2 in clinical samples [J].

Amaral, Catarina ;

Antunes, Wilson ;

Moe, Elin ;

Duarte, Americo G. ;

Lima, Luis M. P. ;

Santos, Cristiana ;

Gomes, Ines L. ;

Afonso, Goncalo S. ;

Vieira, Ricardo ;

Teles, Helena Sofia S. ;

Reis, Marisa S. ;

Ramalho da Silva, Manuel A. ;

Henriques, Ana Margarida ;

Fevereiro, Miguel ;

Ventura, M. Rita ;

Serrano, Monica ;

Pimentel, Catarina .

SCIENTIFIC REPORTS, 2021, 11 (01)

[2] Colorimetric RT-LAMP SARS-CoV-2 diagnostic sensitivity relies on color interpretation and viral load [J].

Aoki, Mateus Nobrega ;

Coelho, Bruna de Oliveira ;

Bentim Goes, Luiz Gustavo ;

Minoprio, Paola ;

Durigon, Edison Luiz ;

Morello, Luis Gustavo ;

Marchini, Fabricio Klerynton ;

Riediger, Irina Natassja ;

Debur, Maria do Carmo ;

Nakaya, Helder I. ;

Blanes, Lucas .

SCIENTIFIC REPORTS, 2021, 11 (01)

[3] A Saliva-Based RNA Extraction-Free Workflow Integrated With Cas13a for SARS-CoV-2 Detection [J].

Azmi, Iqbal ;

Faizan, Md Imam ;

Kumar, Rohit ;

Raj Yadav, Siddharth ;

Chaudhary, Nisha ;

Kumar Singh, Deepak ;

Butola, Ruchika ;

Ganotra, Aryan ;

Datt Joshi, Gopal ;

Deep Jhingan, Gagan ;

Iqbal, Jawed ;

Joshi, Mohan C. ;

Ahmad, Tanveer .

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2021, 11

[4]

Flynn M.J., 2020, MEDRXIV, DOI DOI 10.1101/2020.11.19.20234948

[5] Probe-free label system for rapid detection of Cronobacter genus in powdered infant formula [J].

Fu, Shiqian ;

Jiang, Yujun ;

Jiang, Xia ;

Zhao, Yueming ;

Chen, Sihan ;

Yang, Xinyan ;

Man, Chaoxin .

AMB EXPRESS, 2018, 8

[6] Progress in loop-mediated isothermal amplification assay for detection of Schistosoma mansoni DNA: towards a ready-to-use test [J].

Garcia-Bernalt Diego, J. ;

Fernandez-Soto, P. ;

Crego-Vicente, B. ;

Alonso-Castrillejo, S. ;

Febrer-Sendra, B. ;

Gomez-Sanchez, A. ;

Vicente, B. ;

Lopez-Aban, J. ;

Muro, A. .

SCIENTIFIC REPORTS, 2019, 9 (1)

[7] Loop-mediated isothermal amplification for the detection of SARS-CoV-2 in saliva [J].

Janikova, Monika ;

Hodosy, Julius ;

Boor, Peter ;

Klempa, Boris ;

Celec, Peter .

MICROBIAL BIOTECHNOLOGY, 2021, 14 (01) :307-316

[8] Rapid and Extraction-Free Detection of SARS-CoV-2 from Saliva by Colorimetric Reverse-Transcription Loop-Mediated Isothermal Amplification [J].

Lalli, Matthew A. ;

Langmade, S. Joshua ;

Chen, Xuhua ;

Fronick, Catrina C. ;

Sawyer, Christopher S. ;

Burcea, Lauren C. ;

Wilkinson, Michael N. ;

Fulton, Robert S. ;

Heinz, Michael ;

Buchser, William J. ;

Head, Richard D. ;

Mitra, Robi D. ;

Milbrandt, Jeffrey .

CLINICAL CHEMISTRY, 2021, 67 (02) :415-424

[9] Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2 [J].

Lau, Yee Ling ;

Ismail, Ilyiana ;

Mustapa, Nur Izati ;

Lai, Meng Yee ;

Soh, Tuan Suhaila Tuan ;

Hassan, Afifah ;

Peariasamy, Kalaiarasu M. ;

Lee, Yee Leng ;

Chong, Yoong Min ;

Sam, I-Ching ;

Goh, Pik Pin .

PEERJ, 2020, 8

[10] Loop-mediated isothermal amplification of DNA [J].

Notomi, Tsugunori ;

Okayama, Hiroto ;

Masubuchi, Harumi ;

Yonekawa, Toshihiro ;

Watanabe, Keiko ;

Amino, Nobuyuki ;

Hase, Tetsu .

NUCLEIC ACIDS RESEARCH, 2000, 28 (12)

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