Cloning and analysis of Nile tilapia Toll-like receptors type-3 mRNA

被引：14

作者：

Abouelmaatti, Reham R. ^{[1
,2
,3
]}

Algammal, Abdelazeem M. ^{[4
]}

Li, Xiaokun ^{[1
,2
]}

Ma, Jisheng ^{[1
,2
]}

Abdelnaby, Eman A. ^{[5
,6
]}

Elfeil, Wael M. K. ^{[7
,8
]}

机构：

[1] Jilin Univ, Dept Biochem, Norman Bethune Coll Med, Changchun 130021, Jilin, Peoples R China

[2] Wenzhou Med Univ, Sch Pharm, Wenzhou, Zhejiang, Peoples R China

[3] Minist Agr, Gen Org Vet Serv, Sharkia Vet Directorate, Key Lab Anim Epidemiol & Zoonosis, Cairo, Egypt

[4] Suez Canal Univ, Fac Vet Med, Bacteriol Immunol & Mycol Dept, Ismailia, Egypt

[5] Suez Canal Univ, Fac Vet Med, Dept Pharmacol, Ismailia, Egypt

[6] Tokyo Univ Agr & Technol Fuchu, Tokyo, Japan

[7] Jilin Univ, Coll Vet Med & Anim Sci, Changchun 130021, Jilin, Peoples R China

[8] Suez Canal Univ, Fac Vet Med, Poultry & Rabbit Med Dept, Ismailia, Egypt

来源：

CENTRAL EUROPEAN JOURNAL OF IMMUNOLOGY | 2013年 / 38卷 / 03期

关键词：

Nile tilapia; single nucleotide polymorphisms; fish; Toll-like receptor 3-TLR-3; gene expression; PATHOGEN RECOGNITION RECEPTORS; MOLECULAR-CLONING; EXPRESSION ANALYSIS; IFN-GAMMA; CHICKEN; GENE; IDENTIFICATION; EVOLUTION; TLR4; PHYLOGENY;

D O I：

10.5114/ceji.2013.37740

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Toll-like receptors (TLRs) are the best understood of the innate immune receptors that detect infections in vertebrates. However the fish TLRs also exhibit very distinct features and a large diversity, which is likely derived from their diverse evolutionary history and the distinct environments that they occupy. Little is known about the fish immune system structure. Our work was aimed to identify and clone the Nile tilapia TLR-3 as a model of fresh water fish species; we cloned the full-length cDNA sequence of Nile tilapia (Oreochromis niloticus) TLR-3 and according to our knowledge, it is the first report illustrating tilapia TLR-3. The complete cDNA sequence of Nile tilapia TLR-3 was 2736 pair base and it encodes a polypeptide of 912 amino acids. Analysis of the deduced amino acid sequence indicated that Nile tilapia TLR-3 has typical structural features and main component of proteins belonging to the TLR family. Our results illustrate a complete and functional Nile tilapia TLR-3 and it is considered an ortholog of the other vertebrate's receptor

引用

页码：277 / 282

页数：6

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