Carbohydrate analyses with high-performance anion-exchange chromatography

被引：197
作者：
Lee, YC
机构：
[1] Biology Department, Johns Hopkins University, Baltimore, MD 21218
来源：
JOURNAL OF CHROMATOGRAPHY A | 1996年 / 720卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(95)00222-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-performance anion-exchange chromatography (HPAEC), in tandem with pulsed amperometric detector (PAD) is a powerful tool for carbohydrate analysis. It requires no pre- or postcolumn derivatization, and yet offers superb resolution and sensitivity. In its basic mode, alcoxide anions from the hydroxyl groups of carbohydrates as well as other anionic substituens are utilized. In addition to the number of hydroxyl groups, separation can be based on anomers, positional isomers, as well as degree of polymerization.
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页码：137 / 149
页数：13
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