Metabolic Activation of Heterocyclic Amines and Expression of Xenobiotic-Metabolizing Enzymes in the Gastrointestinal Tract of Rats

被引:3
作者
Darwish, Wageh S. [1 ,2 ]
Nakayama, Shouta M. M. [1 ]
Itotani, Yuumi [1 ]
Ohno, Marumi [1 ]
Ikenaka, Yoshinori [1 ]
Ishizuka, Mayumi [1 ]
机构
[1] Hokkaido Univ, Lab Toxicol, Dept Environm Vet Sci, Grad Sch Vet Med,Kita Ku, Sapporo, Hokkaido 0600818, Japan
[2] Zagazig Univ, Food Control Dept, Fac Vet Med, Zagazig 44510, Egypt
基金
日本学术振兴会;
关键词
gastrointestinal tract; heterocyclic amines; xenobiotic-metabolizing enzymes; AROMATIC-AMINES; IN-VITRO; GENETIC SUSCEPTIBILITY; SMALL-INTESTINE; HUMAN CANCER; RISK; INDUCTION; EXPOSURE; 2-AMINO-1-METHYL-6-PHENYLIMIDAZO<4,5-B>PYRIDINE; DETOXIFICATION;
D O I
10.1111/1750-3841.12931
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Heterocyclic amines get entry into human body mainly through ingestion of pan-fried meats cooked at high temperatures. Exposure of the gastrointestinal tract (GIT) to ingested xenobiotics prior to delivery to the liver may lead to metabolic activation, which may explain the high incidence of GIT carcinogenesis. Therefore, this study investigated the mutagenic activation of 2 heterocyclic amines, 2-aminoanthracene (2-AA) and 3-amino-1-methyl-5H-prydo[4,3-b]indole (Trp-P-2), in the GIT of rats. In addition, the constitutive mRNA expression profiles of xenobiotic-metabolizing enzymes (XMEs) in the GIT of rats were examined. Metabolic activation of 2-AA was detected in all GIT tissues except the duodenum and rectum, and it was detected at high levels in the ileum and cecum. Furthermore, we revealed high metabolic activation of 2-AA and Trp-P-2 in the jejunum. The mRNA expression of phase I and II enzymes in rat GIT corresponded with their mutagenic activation ability. In conclusion, our results suggest that different expression levels of XME among GIT tissues may contribute to the tissue-specific differences in metabolic activation of xenobiotics such as heterocyclic amines in rats. Practical Application This study declares mutagenic activation of 2 heterocyclic amines namely 2-aminoanthracene (2-AA) and 3-amino-1-methyl-5H-prydo[4,3-b]indole (Trp-P-2), in the gastrointestinal tract (GIT) of rats. In addition, results obtained in this study suggest that GIT tissue-specific expression of xenobiotic metabolizing enzymes may contribute to the tissue-specific mutagenesis/carcinogenesis.
引用
收藏
页码:T1627 / T1632
页数:6
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