Fucoidan inhibits activation and receptor binding of transforming growth factor-β1

被引：23
作者：
Kim, Tae Hee [1 ]
Lee, Eun Kyoung [2 ]
Lee, Mee Jeong [3 ]
Kim, Ji Hyun [1 ]
Yang, Won Seok [1 ]
机构：
[1] Univ Ulsan, Coll Med, Asan Med Ctr, Div Nephrol,Dept Internal Med, Seoul 138736, South Korea
[2] Dankook Univ, Coll Med, Dept Internal Med, Cheonan 330715, South Korea
[3] Dankook Univ, Coll Med, Dept Pediat, Cheonan 330715, South Korea
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 432卷 / 01期
关键词：
Fucoidan; Furin-like proprotein convertase; Latent transforming growth factor-beta 1; Transforming growth factor-beta 1; GROWTH-FACTOR-BETA; TGF-BETA; MYOCARDIAL-ISCHEMIA; IN-VITRO; LATENT; POLYSACCHARIDE; PROLIFERATION; ADHESION; HEPARIN; FUCANS;
D O I：
10.1016/j.bbrc.2013.01.055
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fucoidan, a sulfated, fucose-rich polysaccharide isolated from marine brown algae, has antifibrotic effects. We investigated the biologic effects of interactions of fucoidan with transforming growth factor-beta 1 (TGF-beta 1) and latent TGF-beta 1 (LTGF-beta 1). TGF-beta 1 bound to fucoidan was unable to interact with its reteptor. In agreement with this, fucoidan attenuated the cellular effect of TGF-beta 1 as measured by phosphorylation of Smad2. Binding of fucoidan rendered LTGF-beta 1 resistant to activation as follows. Fucoidan inhibited furin-like proprotein convertase-mediated activation of platelet LTGF-beta 1 without suppression of the enzyme. In addition, acid- or heat-activation of small recombinant LTGF-beta 1 and acid-activation of large LTGF-beta 1 in cultured cell supernatant were also inhibited by fucoidan. Fucoidan is a mixture of polysaccharides of different sizes. As molecular weight of fucoidan increases, it had more inhibitory effects on TGF-beta 1 and LTGF-beta 1. In conclusion, inhibitions of LTGF-beta 1 activation and TGF-beta 1 receptor binding by fucoidan may in part account for its antifibrotic effects. (C) 2013 Elsevier Inc. All rights reserved.
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收藏
页码：163 / 168
页数：6
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