Sustaining Interferon Induction by a High-Passage Atypical Porcine Reproductive and Respiratory Syndrome Virus Strain

被引：9

作者：

Ma, Zexu ^{[1
]}

Yu, Ying ^{[1
]}

Xiao, Yueqiang ^{[1
]}

Opriessnig, Tanja ^{[2
,3
,4
]}

Wang, Rong ^{[1
]}

Yang, Liping ^{[1
]}

Nan, Yuchen ^{[1
]}

Samal, Siba K. ^{[5
]}

Halbur, Patrick G. ^{[4
]}

Zhang, Yan-Jin ^{[1
]}

机构：

[1] Univ Maryland, VA MD Coll Vet Med, Mol Virol Lab, College Pk, MD 20742 USA

[2] Univ Edinburgh, Roslin Inst, Roslin, Midlothian, Scotland

[3] Univ Edinburgh, Royal Dick Sch Vet Studies, Roslin, Midlothian, Scotland

[4] Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA

[5] Univ Maryland, VA MD Coll Vet Med, Virol Lab, College Pk, MD 20742 USA

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

IN-VITRO; PASSIVE TRANSFER; PRRSV; REPLICATION; ANTIBODIES; INFECTION; IDENTIFICATION; PATHOGENICITY; PATHOGENESIS; PROTECTION;

D O I：

10.1038/srep36312

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Porcine reproductive and respiratory syndrome virus (PRRSV) strain A2MC2 induces type I interferons in cultured cells. The objective of this study was to attenuate this strain by serial passaging in MARC-145 cells and assess its virulence and immunogenicity in pigs. The A2MC2 serially passaged 90 times (A2MC2-P90) retains the feature of interferon induction. The A2MC2-P90 replicates faster with a higher virus yield than wild type A2MC2 virus. Infection of primary pulmonary alveolar macrophages (PAMs) also induces interferons. Sequence analysis showed that the A2MC2-P90 has genomic nucleic acid identity of 99.8% to the wild type but has a deletion of 543 nucleotides in nsp2. The deletion occurred in passage 60. The A2MC2-P90 genome has a total of 35 nucleotide variations from the wild type, leading to 26 amino acid differences. Inoculation of three-week-old piglets showed that A2MC2-P90 is avirulent and elicits immune response. Compared with Ingelvac PRRS (R) MLV strain, A2MC2-P90 elicits higher virus neutralizing antibodies. The attenuated IFN-inducing A2MC2-P90 should be useful for development of an improved PRRSV vaccine.

引用

页数：10

共 36 条

[1] Interferon-α response to swine arterivirus (PoAV), the porcine reproductive and respiratory syndrome virus [J].

Albina, E ;

Carrat, C ;

Charley, B .

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 1998, 18 (07) :485-490

[2] Adenovirus-Mediated Expression of Interferon-α Delays Viral Replication and Reduces Disease Signs in Swine Challenged with Porcine Reproductive and Respiratory Syndrome Virus [J].

Brockmeier, S. L. ;

Lager, K. M. ;

Grubman, M. J. ;

Brough, D. E. ;

Ettyreddy, D. ;

Sacco, R. E. ;

Gauger, P. C. ;

Loving, C. L. ;

Vorwald, A. C. ;

Kehrli, M. E., Jr. ;

Lehmkuhl, H. D. .

VIRAL IMMUNOLOGY, 2009, 22 (03) :173-180

[3] The Presence of Alpha Interferon at the Time of Infection Alters the Innate and Adaptive Immune Responses to Porcine Reproductive and Respiratory Syndrome Virus [J].

Brockmeier, Susan L. ;

Loving, Crystal L. ;

Nelson, Eric A. ;

Miller, Laura C. ;

Nicholson, Tracy L. ;

Grubman, Marvin J. ;

Brough, Douglas E. ;

Kehrli, Marcus E., Jr. .

CLINICAL AND VACCINE IMMUNOLOGY, 2012, 19 (04) :508-514

[4]

Buddaert W, 1998, ADV EXP MED BIOL, V440, P461

[5] GP3 is a structural component of the PRRSV type II (US) virion [J].

de Lima, M. ;

Ansari, I. H. ;

Das, P. B. ;

Ku, B. J. ;

Martinez-Lobo, F. J. ;

Pattnaik, A. K. ;

Osorio, F. A. .

VIROLOGY, 2009, 390 (01) :31-36

[6]

Faaberg K. S., 2012, VIRUS TAXONOMY CLASS

[7] The PRRSV replicase: Exploring the multifunctionality of an intriguing set of nonstructural proteins [J].

Fang, Ying ;

Snijder, Eric J. .

VIRUS RESEARCH, 2010, 154 (1-2) :61-76

[8] Immunomodulatory functions of type I interferons [J].

Gonzalez-Navajas, Jose M. ;

Lee, Jongdae ;

David, Michael ;

Raz, Eyal .

NATURE REVIEWS IMMUNOLOGY, 2012, 12 (02) :125-135

[9] COMPARISON OF THE PATHOGENICITY OF 2 US PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS ISOLATES WITH THAT OF THE LELYSTAD VIRUS [J].

HALBUR, PG ;

PAUL, PS ;

FREY, ML ;

LANDGRAF, J ;

EERNISSE, K ;

MENG, XJ ;

LUM, MA ;

ANDREWS, JJ ;

RATHJE, JA .

VETERINARY PATHOLOGY, 1995, 32 (06) :648-660

[10] Comparative pathogenicity of nine US porcine reproductive and respiratory syndrome virus (PRRSV) isolates in a five-week-old cesarean-derived, colostrum-deprived pig model [J].

Halbur, PG ;

Paul, PS ;

Meng, XJ ;

Lum, MA ;

Andrews, JJ ;

Rathje, JA .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1996, 8 (01) :11-20

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