Tentative exploration of pharmacodynamic substances: Pharmacological effects, chemical compositions, and multi-components pharmacokinetic characteristics of ESZWD in CHF-HKYd rats

被引：5
作者：
Hong, Li-li [1 ,2 ,3 ,4 ]
Zhao, Yan [1 ,2 ,3 ,4 ]
Chen, Wei-Dong [1 ,2 ,3 ,4 ]
Yang, Chen-Yu [1 ,2 ,3 ,4 ]
Li, Guo-Zhuan [1 ,2 ,3 ,4 ]
Wang, Hong-Song [1 ,2 ,3 ,4 ]
Cheng, Xiao-Yu [5 ]
机构：
[1] Anhui Univ Chinese Med, Sch Pharm, Hefei, Peoples R China
[2] Anhui Univ Chinese Med, Sch Pharm, Anhui Prov Key Lab Chinese Med Formula, Hefei, Peoples R China
[3] Anhui Univ Chinese Med, Synerget Innovat Ctr Anhui Authent Chinese Med Qua, Sch Pharm, Hefei, Peoples R China
[4] Anhui Univ Chinese Med, Sch Pharm, MOE Anhui Joint Collaborat Innovat Ctr Qual Improv, Hefei, Peoples R China
[5] Anhui Univ Tradit Chinese Med, Anhui Univ Chinese Med, Affiliated Hosp 1, Dept Geriatr Cardiol, Hefei, Peoples R China
来源：
FRONTIERS IN CARDIOVASCULAR MEDICINE | 2022年 / 9卷
关键词：
Ershen Zhenwu Decoction; chronic heart failure with heart-kidney Yang deficiency; UPLC; Q-TOF-MS; pharmacokinetic; pharmacodynamic substances; CHRONIC HEART-FAILURE; OXIDATIVE STRESS; MILTIRONE; PAEONIFLORIN;
D O I：
10.3389/fcvm.2022.913661
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The chemical components of Xin'an famous prescription Ershen Zhenwu Decoction (ESZWD) are still unclear. The results showed that ESZWD could significantly reduce left ventricular end diastolic diameter, decrease N-terminal pro-brain natriuretic peptide (NT-proBNP), angiotensinII, aldosterone, reactive oxygen species, and malondialdehyde, increase serum superoxide dismutase, while had no significant effect on inflammatory factors. Ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS) analysis detected 30 prototype components in model rats' serum, mainly including alkaloids, saponins, terpenoids, tanshinones, phenols. UPLC-MS/MS successfully detected the pharmacokinetic parameters of four components, and correlation analysis shows that there are negative correlations between four compounds and serum NT-proBNP. Thirty components of ESZWD may play a therapeutic role in chronic heart failure with heart-kidney Yang deficiency (CHF-HKYd) by improving myocardial injury, reducing oxidative stress levels, and inhibiting activation of the RAAS system in rats. Salsolinol, aconitine, paeoniflorin, and miltrione are equipped with potential characteristics as pharmacodynamic substances for ESZWD in treating CHF-HKYd. Additionally, the constituents of ESZWD in CHF-HKYd rats are different from normal rats, which provided a reference for the selection of subjects for further study.
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页数：14
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