Polymerase chain reaction for diagnosis and identification of distinct variants of Crimean-Congo hemorrhagic fever virus in the United Arab Emirates

被引:83
作者
Schwarz, TF
Nsanze, H
Longson, M
Nitschko, H
Gilch, S
Shurie, H
Ameen, A
Zahir, ARM
Acharya, UG
Jager, G
机构
[1] UNIV MUNICH, MAX VON PETTENKOFER INST HYG & MED MICROBIOL, MUNICH, GERMANY
[2] UNITED ARAB EMIRATES UNIV, FAC MED & HLTH SCI, DEPT MED MICROBIOL, AL AIN, U ARAB EMIRATES
关键词
D O I
10.4269/ajtmh.1996.55.190
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Viral hemorrhagic fever has re-emerged in the United Arab Emirates (UAE) since November 1993. Genomic RNA of Crimean-Congo hemorrhagic virus (C-CHFV) was detected by a newly developed, nested reverse transcriptase polymerase chain reaction (RT-PCR) in the sera of four (25.0%) of 16 suspected cases of viral hemorrhagic fever. The RT-PCR was based on oligonucleotide primers deducted from the small RNA segment encoding the nucleoprotein of the virus. By comparison with a nucleotide sequence of a C-CHFV isolate from a Chinese sheep, a divergence of 10.0-11.8% was detected in the C-CHFV variants causing the UAE outbreak. In the four positive sera, three phylogenetically distinct C-CHFV variants were amplified and confirmed by direct sequencing of the PCR fragments. These C-CHFV sequences were obtained directly from sera of infected humans without prior propagation in cell culture. The RT-PCR allows rapid detection of genomic C-CHFV RNA in clinical specimens and study of the molecular epidemiology of this infection.
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页码:190 / 196
页数:7
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