Natural occurrence of White spot syndrome virus and Infectious hypodermal and hematopoietic necrosis virus in Neohelice granulata crab

被引：21

作者：

Cavalli, Lissandra Souto ^{[1
]}

Batista, Carolina Reyes ^{[2
]}

Nornberg, Bruna F. S. ^{[2
]}

Mayer, Fabiana Quoos ^{[1
]}

Seixas, Fabiana K. ^{[3
]}

Romano, Luis Alberto ^{[4
]}

Marins, Luis Fernando ^{[2
]}

Abreu, Paulo Cesar ^{[4
]}

机构：

[1] Fundacao Estadual Pesquisa Agr FEPAGRO Saude Anim, Inst Pesquisas Vet Desiderio Finamor, BR-92990000 Eldorado Do Sul, RS, Brazil

[2] Univ Fed Rio Grande, Inst Ciencias Biol, Rio Grande, Brazil

[3] Univ Fed Pelotas, Ctr Desenvolvimento Tecnol, Pelotas, RS, Brazil

[4] Univ Fed Rio Grande, Inst Oceanog, Rio Grande, Brazil

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2013年 / 114卷 / 01期

关键词：

WSSV; IHHNV; Neohelice granulata; Viral disease; Bioindicator; PRAWN PENAEUS-MONODON; CHASMAGNATHUS-GRANULATA; SHRIMP; DISEASES; MORPHOGENESIS; STYLIROSTRIS; PURIFICATION; PREVALENCE; EMERGENCE; IMPACT;

D O I：

10.1016/j.jip.2013.06.002

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

White spot syndrome virus (WSSV) and Infectious hypodermal and hematopoietic necrosis virus (IHHNV) are two infectious agents associated to economic losses in shrimp aquaculture. As virus spread occurs through vectors and hosts, this study sought to verify the presence of WSSV and IHHNV in Neohelice granulata crab from Lagoa dos Patos estuary in Brazil and nearby shrimp farms. DNA extractions were performed with phenol/chloroform protocol. Molecular diagnosis was carried out by nested PCR for WSSV and one-step PCR for IHHNV. Results showed the presence of WSSV on crabs of both Lagoa dos Patos and farms, while IHHNV was found only on crabs collected in estuary. This is the first study to report IHHNV presence in N. granulata. Moreover, as analyzed crabs had no clinical symptoms or showed in situ mortality, we suggest its use as a bioindicator for virus occurrence in aquatic environments. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：86 / 88

页数：3

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