Enzyme-linked antibody aptamer assays based colorimetric detection of soluble fraction of activated leukocyte cell adhesion molecule

被引：12
作者：
Her, Jin [1 ]
Jo, Hunho [1 ]
Ban, Changill [1 ]
机构：
[1] Pohang Univ Sci & Technol, Dept Chem, 77 Cheongam Ro, Pohang 790784, Gyeongbuk, South Korea
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2017年 / 242卷
基金：
新加坡国家研究基金会;
关键词：
Aptamer; ELAA assay; ALCAM; CD166; PLASMON RESONANCE SPECTROSCOPY; CANCER; CARCINOMA; CD166/ALCAM; ALCAM/CD166; SELEX;
D O I：
10.1016/j.snb.2016.11.070
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Patients with pancreatic and colorectal cancers have elevated levels of activated leukocyte cell adhesion molecule (ALCAM) in their blood compared to healthy individuals. To this end, sensitive detection of soluble fraction of ALCAM (sALCAM), also called CD166, was performed using enzyme-linked antibody aptamer (ELAA) assay. For the assay, aptamer specific for sALCAM was used as a capturing probe and polyclonal antibody modified with an enzyme was used as a detecting probe for colorimetric visualization. The ELAA assay was able to detect sALCAM as low as 0.31 ng/mL. In addition, the assay enabled the differentiation of sALCAM from other proteins in buffer as well as in human serum. The ELAA assay provides cost-effective, highly sensitive, and reproducible detection of sALCAM. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：529 / 534
页数：6
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