A triallelic system of S5 is a major regulator of the reproductive barrier and compatibility of indica-japonica hybrids in rice

被引：241

作者：

Chen, Jiongjiong ^{[1
,2
]}

Ding, Jihua ^{[1
,2
]}

Ouyang, Yidan ^{[1
,2
]}

Du, Hongyi ^{[1
,2
]}

Yang, Jiangyi ^{[1
,2
]}

Cheng, Ke ^{[1
,2
]}

Zhao, Jie ^{[3
]}

Qiu, Shuqing ^{[1
,2
]}

Zhang, Xuelian ^{[3
]}

Yao, Jialing ^{[1
,2
]}

Liu, Kede ^{[1
,2
]}

Wang, Lei ^{[1
,2
]}

Xu, Caiguo ^{[1
,2
]}

Li, Xianghua ^{[1
,2
]}

Xue, Yongbiao ^{[4
]}

Xia, Mian ^{[5
]}

Ji, Qing ^{[6
]}

Lu, Jufei ^{[6
]}

Xu, Mingliang ^{[7
]}

Zhang, Qifa ^{[1
,2
]}

机构：

[1] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

[2] Huazhong Agr Univ, Natl Ctr Plant Gene Res, Wuhan 430070, Peoples R China

[3] Wuhan Univ, Coll Life Sci, Key Lab Minist Educ Plant Dev Biol, Wuhan 430072, Peoples R China

[4] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

[5] China Bioway Biotech Grp, Natl Ctr Crop Design, Beijing 100085, Peoples R China

[6] Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China

[7] China Agr Univ, Natl Maize Improvement Ctr, Beijing 100094, Peoples R China

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 32期

基金：

中国国家自然科学基金;

关键词：

subspecies of rice; hybrid sterility; wide compatibility; aspartic protease;

D O I：

10.1073/pnas.0804761105

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Hybrid sterility is a major form of postzygotic reproductive isolation. Although reproductive isolation has been a key issue in evolutionary biology for many decades in a wide range of organisms, only very recently a few genes for reproductive isolation were identified. The Asian cultivated rice (Oryza sativa L.) is divided into two subspecies, indica and japonica. Hybrids between indica and japonica varieties are usually highly sterile. A special group of rice germplasm, referred to as wide-compatibility varieties, is able to produce highly fertile hybrids when crossed to both indica and japonica. In this study, we cloned S5, a major locus for indica-japonica hybrid sterility and wide compatibility, using a map-based cloning approach. We show that S5 encodes an aspartic protease conditioning embryo-sac fertility. The indica (S5-i) and japonica (S5-j) alleles differ by two nucleotides. The wide compatibility gene (S5-n) has a large deletion in the IN terminus of the predicted S5 protein, causing subcellular mislocalization of the protein, and thus is presumably nonfunctional. This triallelic system has a profound implication in the evolution and artificial breeding of cultivated rice. Genetic differentiation between indica and japonica would have been enforced because of the reproductive barrier caused by S5-i and S5-j, and species coherence would have been maintained by gene flow enabled by the wide compatibility gene.

引用

页码：11436 / 11441

页数：6

共 43 条

[1] The rice nucellin gene ortholog OsAsp1 encodes an active aspartic protease without a plant-specific insert and is strongly expressed in early embryo [J].