3-(10'-Phenothiazinyl)propionic acid is a potent primary enhancer of peroxidase-induced chemiluminescence and its application in sensitive ELISA of methylglyoxal-modified low density lipoprotein

被引：35

作者：

Sakharov, Ivan Yu ^{[1
]}

Demiyanova, Alexandra S. ^{[1
]}

Gribas, Anastasia V. ^{[2
]}

Uskova, Natalia A. ^{[1
]}

Efremov, Evgeny E. ^{[3
]}

Vdovenko, Marina M. ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia

[2] Plekhanov Russian Univ Econ, Moscow 115998, Russia

[3] Russian Cardiol Res Ctr, Inst Expt Cardiol, Moscow 121552, Russia

来源：

TALANTA | 2013年 / 115卷

基金：

俄罗斯基础研究基金会;

关键词：

3-(10'-Phenothiazinyl)-propinonic acid; Chemiluminescence; Enhancement; Peroxidase; Enzyme immunoassay; Methylglyoxal-modified low density; lipoprotein; HORSERADISH-PEROXIDASE; ENZYME IMMUNOASSAYS; LUMINOL; LUMINESCENCE; MECHANISM; ASSAY;

D O I：

10.1016/j.talanta.2013.05.074

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Using a full factorial design the optimization of experimental conditions of enhanced chemiluminescence reaction (ECR) catalyzed by horseradish peroxidase (HRP) in the presence of 3-(10'-phenothiazinyl) propionic acid (PPA) as a primary enhancer was performed. The effect of concentrations of PPA, hydrogen peroxide, MORPH, luminol, and Tris on a ratio of peroxidase-catalyzed CL to background was studied. The detection limit value of HRP in ECR with PPA was 0.09 pM. Using PPA the ultra-sensitive chemiluminescent ELISA for determination of methylglyoxal-modified low density lipoprotein was developed. The detection limit value for the developed method was 0.5 ng mL(-1). The obtained results open up very promising perspectives for using PPA to improve the sensitivity of enzyme immunoassay kits. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：414 / 417

页数：4