Monitoring β-Secretase Activity in Living Cells with a Membrane-Anchored FRET Probe

被引：29

作者：

Folk, Drew S. ^{[1
]}

Torosian, Justin C. ^{[1
]}

Hwang, Sunhee ^{[1
]}

McCafferty, Dewey G. ^{[1
]}

Franz, Katherine J. ^{[1
]}

机构：

[1] Duke Univ, Dept Chem, Durham, NC 27708 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 43期

基金：

美国国家卫生研究院;

关键词：

Alzheimer's disease; analytical methods; enzymes; Forster resonance energy transfer (FRET); imaging agents; AMYLOID PRECURSOR PROTEIN; ALZHEIMERS-DISEASE; POTENT INHIBITORS; BACE-1; INHIBITORS; ALPHA-SECRETASE; LIPID RAFTS; IN-VITRO; DESIGN; OLIGOMERIZATION; CLEAVAGE;

D O I：

10.1002/anie.201206673

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Turn it on! β-MAP is a sensitive FRET probe with specificity for monitoring the enzyme β-secretase (BACE), which is associated with Alzheimer's disease. After hydrolysis by the enzyme BACE, the probe fluoresces and thus allows real-time spatial and temporal assessment of enzymatic activity in living cells. β-MAP was used to confirm the cellular efficacy of a reported inhibitor without the need for mutated cell lines or antibodies. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

收藏

页码：10795 / 10799

页数：5

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