Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke

被引：16
作者：
He, Dongsheng [1 ,2 ,3 ,5 ,6 ]
Zhang, Zhuo [4 ]
Lao, Jiamin [1 ,2 ,3 ,5 ,6 ]
Meng, Hailan [1 ,2 ,3 ,5 ,6 ]
Han, Lijuan [1 ,2 ,3 ,5 ,6 ]
Chen, Fan [1 ,2 ,3 ,5 ,6 ]
Ye, Dan [1 ,2 ,3 ,5 ,6 ]
Zhang, He [1 ,2 ,3 ,5 ,6 ]
Xu, Yun [1 ,2 ,3 ,5 ,6 ]
机构：
[1] Nanjing Univ, Dept Neurol, Sch Med, Affiliated Drum Tower Hosp, Nanjing 210008, Jiangsu, Peoples R China
[2] Nanjing Univ, Jiangsu Key Lab Mol Med, Sch Med, Nanjing 210008, Jiangsu, Peoples R China
[3] Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Nanjing 210008, Jiangsu, Peoples R China
[4] Nanjing Med Univ, Childrens Hosp Nanjing, Dept Gastroenterol, Nanjing 210008, Jiangsu, Peoples R China
[5] Jiangsu Prov Stroke Ctr Diag & Therapy, Nanjing 210008, Jiangsu, Peoples R China
[6] Nanjing Neuropsychiat Clin, Med Ctr, Nanjing 210008, Jiangsu, Peoples R China
来源：
AGING AND DISEASE | 2016年 / 7卷 / 05期
基金：
中国国家自然科学基金;
关键词：
stroke; hUC-MSCs; proteomics; iTRAQ; transplantation; human; STE20-LIKE KINASE SLK; PROSTAGLANDIN-D SYNTHASE; HUMAN BREAST-CANCER; ANNEXIN A6; ISCHEMIC-STROKE; GENE; GRB2; PROTEIN; EXPRESSION; DIFFERENTIATION;
D O I：
10.14336/AD.2016.0121
中图分类号：
R592 [老年病学]; C [社会科学总论];
学科分类号：
03 ; 0303 ; 100203 ;
摘要：
Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.
引用
收藏
页码：623 / 634
页数：12
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