Insights into structural and regulatory roles of Sec16 in COPII vesicle formation at ER exit sites

被引:66
作者
Yorimitsu, Tomohiro [1 ]
Sato, Ken [1 ]
机构
[1] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Meguro Ku, Tokyo 1538902, Japan
基金
日本学术振兴会;
关键词
ENDOPLASMIC-RETICULUM; SUBUNIT INTERACTIONS; PROTEIN SECRETION; COAT; TRANSPORT; ENCODES; GTP; REQUIREMENTS; LOCALIZATION; BINDING;
D O I
10.1091/mbc.E12-05-0356
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
COPII-coated buds are formed at endoplasmic reticulum exit sites (ERES) to mediate ER-to-Golgi transport. Sec16 is an essential factor in ERES formation, as well as in COPII-mediated traffic in vivo. Sec16 interacts with multiple COPII proteins, although the functional significance of these interactions remains unknown. Here we present evidence that full-length Sec16 plays an important role in regulating Sar1 GTPase activity at the late steps of COPII vesicle formation. We show that Sec16 interacts with Sec23 and Sar1 through its C-terminal conserved region and hinders the ability of Sec31 to stimulate Sec23 GAP activity toward Sar1. We also find that purified Sec16 alone can self-assemble into homo-oligomeric complexes on a planar lipid membrane. These features ensure prolonged COPII coat association within a preformed Sec16 cluster, which may lead to the formation of ERES. Our results indicate a mechanistic relationship between COPII coat assembly and ERES formation.
引用
收藏
页码:2930 / 2942
页数:13
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