PRODUCTION OF LIGNINOLYTIC ENZYMES BY SOLID-STATE FERMENTATION USING Pleurotus eryngii

被引：28

作者：

Akpinar, Merve ^{[2
]}

Urek, Raziye Ozturk ^{[1
]}

机构：

[1] Dokuz Eylul Univ, Fac Sci, Dept Chem, Div Biochem, TR-35160 Izmir, Turkey

[2] Dokuz Eylul Univ, Grad Sch Nat & Appl Sci, Dept Chem, TR-35160 Izmir, Turkey

来源：

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2012年 / 42卷 / 06期

关键词：

decolorization; grape waste; ligninolytic enzymes; Pleurotus eryngii; solid-state fermentation; ARYL-ALCOHOL OXIDASE; MANGANESE PEROXIDASE; LACCASE ISOENZYMES; DEGRADATION; CULTURES; WASTE; INDUCTION; OSTREATUS; OXIDATION; KINETICS;

D O I：

10.1080/10826068.2012.673528

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Pleurotus eryngii (DC.) Gillet (MCC58) was investigated for its ability to produce various ligninolytic enzymes such as laccase (Lac), manganese peroxidase (MnP), aryl alcohol oxidase (AAO), and lignin peroxidase (LiP) by solid-state fermentation (SSF), which was carried out using a support substrate from the fruit juice industry. The chemical content of grape waste from this industry was studied. Also, the production patterns of these extracellular enzymes were researched during the growth of the organism for a period of 20 days and the protein, reducing sugar, and nitrogen levels were monitored during the stationary cultivation. The highest Lac activity was obtained as 2247.62 +/- 75U/L on day 10 in the presence of 750 mu M Mn2+, while the highest MnP activity was attained as 2198.44 +/- 65U/L on day 15 in the presence of 500 mu M Mn2+. Decolorization of methyl orange and reactive red 2 azo dyes was also achieved with ligninolytic enzymes, produced in SSF of P. eryngii.

引用

页码：582 / 597

页数：16

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