PRODUCTION OF LIGNINOLYTIC ENZYMES BY SOLID-STATE FERMENTATION USING Pleurotus eryngii

被引：28

作者：

Akpinar, Merve ^{[2
]}

Urek, Raziye Ozturk ^{[1
]}

机构：

[1] Dokuz Eylul Univ, Fac Sci, Dept Chem, Div Biochem, TR-35160 Izmir, Turkey

[2] Dokuz Eylul Univ, Grad Sch Nat & Appl Sci, Dept Chem, TR-35160 Izmir, Turkey

来源：

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2012年 / 42卷 / 06期

关键词：

decolorization; grape waste; ligninolytic enzymes; Pleurotus eryngii; solid-state fermentation; ARYL-ALCOHOL OXIDASE; MANGANESE PEROXIDASE; LACCASE ISOENZYMES; DEGRADATION; CULTURES; WASTE; INDUCTION; OSTREATUS; OXIDATION; KINETICS;

D O I：

10.1080/10826068.2012.673528

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Pleurotus eryngii (DC.) Gillet (MCC58) was investigated for its ability to produce various ligninolytic enzymes such as laccase (Lac), manganese peroxidase (MnP), aryl alcohol oxidase (AAO), and lignin peroxidase (LiP) by solid-state fermentation (SSF), which was carried out using a support substrate from the fruit juice industry. The chemical content of grape waste from this industry was studied. Also, the production patterns of these extracellular enzymes were researched during the growth of the organism for a period of 20 days and the protein, reducing sugar, and nitrogen levels were monitored during the stationary cultivation. The highest Lac activity was obtained as 2247.62 +/- 75U/L on day 10 in the presence of 750 mu M Mn2+, while the highest MnP activity was attained as 2198.44 +/- 65U/L on day 15 in the presence of 500 mu M Mn2+. Decolorization of methyl orange and reactive red 2 azo dyes was also achieved with ligninolytic enzymes, produced in SSF of P. eryngii.

引用

页码：582 / 597

页数：16

共 48 条

[1] Potential uses and applications of treated wine waste: a review
Arvanitoyannis, IS
Ladas, D
Mavromatis, A
[J]. INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2006, 41 (05) : 475 - 487
[2] Degradation of lignocellulose by Pleurotus ostreatus in the presence of copper, manganese, lead and zinc
Baldrian, P
Valásková, V
Merhautová, V
Gabriel, J
[J]. RESEARCH IN MICROBIOLOGY, 2005, 156 (5-6) : 670 - 676
[3] Interactions of heavy metals with white-rot fungi
Baldrian, P
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 2003, 32 (01) : 78 - 91
[4] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5] ELICITATION OF LIGNIN BIOSYNTHESIS AND ISOPEROXIDASE ACTIVITY BY PECTIC FRAGMENTS IN SUSPENSION-CULTURES OF CASTOR BEAN
BRUCE, RJ
WEST, CA
[J]. PLANT PHYSIOLOGY, 1989, 91 (03) : 889 - 897
[6] LIGNIN BIODEGRADATION
BUSWELL, JA
ODIER, E
[J]. CRC CRITICAL REVIEWS IN BIOTECHNOLOGY, 1987, 6 (01): : 1 - 60
[7] Comparison between the protease production ability of ligninolytic fungi cultivated in solid state media
Cabaleiro, DR
Rodríguez-Couto, S
Sanromán, A
Longo, MA
[J]. PROCESS BIOCHEMISTRY, 2002, 37 (09) : 1017 - 1023
[8] Description of a versatile peroxidase involved in the natural degradation of lignin that has both manganese peroxidase and lignin peroxidase substrate interaction sites
Camarero, S
Sarkar, S
Ruiz-Dueñas, FJ
Martínez, MJ
Martínez, AT
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (15) : 10324 - 10330
[9] de Oliveira PL, 2009, BRAZ J MICROBIOL, V40, P818, DOI [10.1590/S1517-83822009000400012, 10.1590/S1517-838220090004000012]
[10] Effect of amino acids and vitamins on laccase production by the bird's nest fungus Cyathus bulleri
Dhawan, S
Kuhad, RC
[J]. BIORESOURCE TECHNOLOGY, 2002, 84 (01) : 35 - 38

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