A LAPS array with low cross-talk for non-invasive measurement of cellular metabolism

被引：19

作者：

Hu, Ning ^{[1
]}

Ha, Da ^{[1
]}

Wu, Chengxiong ^{[1
]}

Zhou, Jie ^{[1
]}

Kirsanov, Dmitry ^{[2
]}

Legin, Andrey ^{[2
]}

Wang, Ping ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Biomed Engn, Educ Minist, Biosensor Natl Special Lab,Key Lab Biomed Engn, Hangzhou 310003, Zhejiang, Peoples R China

[2] St Petersburg State Univ, Dept Chem, Lab Chem Sensors, St Petersburg, Russia

来源：

SENSORS AND ACTUATORS A-PHYSICAL | 2012年 / 187卷

基金：

中国国家自然科学基金;

关键词：

Cellular metabolism; Light-addressable potentiometric array; Low cross-talk; Non-invasive measurement; Extracellular acidification; ADDRESSABLE POTENTIOMETRIC SENSOR; ENERGY-METABOLISM; MEMBRANE; MICROPHYSIOMETER; MICROENVIRONMENT; PATHWAY; CHANNEL; GROWTH; ACID;

D O I：

10.1016/j.sna.2012.08.023

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Cellular metabolism is ubiquitous biological mechanism involved in many significant physiological processes, and most of the biological cascade reactions are tightly coupled. Non-invasive measurement of cellular metabolism is important to study the metabolism mechanism and drug effect in a long-term detection. In this study, the two processes of heavy doping and thick oxidation fabricated a new light-ddressable potentiometric sensor array, which was applied to detect the cellular metabolism induced pH change. The performance of the LAPS was tested by the basic characteristic experiments and renal cell experiments. The result showed that the stability and pH resolution of LAPS array were greatly improved. With the development of the sensor design and fabrication, the LAPS will be a utility tool in the field of biological metabolism. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：50 / 56

页数：7

共 35 条

[1] Integrated cell positioning and cell-based ISFET biosensors [J].