Antibacterial activity of chitosan grafting nisin: Preparation and characterization

被引：31

作者：

Zhu, Xiaoming ^{[1
,3
]}

Wu, Huan ^{[2
]}

Yang, Jing ^{[2
]}

Tong, Jun ^{[2
]}

Yi, Jiayan ^{[2
]}

Hu, Zhihai ^{[2
]}

Hu, Jin ^{[2
]}

Wang, Tan ^{[2
]}

Fan, Lihong ^{[1
,2
]}

机构：

[1] Hubei Univ Sci & Technol, Hubei Collaborat Innovat Ctr Nonpower Nucl Techno, Xianning 437100, Peoples R China

[2] Wuhan Univ Technol, Coll Chem Engn, Wuhan 430070, Peoples R China

[3] Hubei Univ Sci & Technol, Sch Nucl Technol & Chem & Biol, Xianning 437100, Peoples R China

来源：

REACTIVE & FUNCTIONAL POLYMERS | 2015年 / 91-92卷

基金：

中国国家自然科学基金;

关键词：

Chitosan; Nisin; Microbial transglutaminase; Antibacterial activity; Low toxicity; COLLAGEN PEPTIDE; FILMS; COPOLYMER;

D O I：

10.1016/j.reactfunctpolym.2015.04.009

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Nisin grafted chitosan was prepared by using microbial transglutaminase as biocatalyst. The transglutaminase-catalyzed reaction displayed high efficiency, high selectivity, mild reaction condition and environmental friendliness. The results revealed that the degree of substitution (DS) of nisin-chitosan could be controlled by adjusting the reaction time, the reaction temperature and the molar ratio of nisin to chitosan. And nisin-chitosan in different pH showed excellent solubility. In addition, in vitro antibacterial activity assessment, nisin-chitosan with the concentration of 0.008 mg/mL showed pronounced inhibitory effect against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis) and Gram-negative bacteria (Escherichia coli). Furthermore, L929 mouse fibroblasts were cultured with nisin-chitosan, and the methylthiazol tetrazolium (MIT) assay showed that nisin-chitosan with the concentration from 0.005 to 0.01 mg/mL displayed low toxicity. The results may contribute to finding the application of nisin-chitosan in pharmaceutical and food industry fields. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：71 / 76

页数：6

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