Label-free DNA sensor construction using self-assembled poly(o-methoxyaniline) hollow nanospheres

被引：15

作者：

Sui, Jing ^{[1
,2
]}

Zhang, Lijuan ^{[1
]}

Peng, Hui ^{[1
,3
]}

机构：

[1] Univ Auckland, Sch Chem Sci, Polymer Elect Res Ctr, Auckland, New Zealand

[2] Qingdao Univ Sci & Technol, Minist Educ, Key Lab Rubber & Plast, Qingdao 266042, Peoples R China

[3] E China Normal Univ, Minist Educ, Key Lab Polar Mat & Devices, Shanghai 200241, Peoples R China

来源：

EUROPEAN POLYMER JOURNAL | 2013年 / 49卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Poly(o-methoxyaniline); Hollow nanospheres; Oligonucleotide sensor; FUNCTIONALIZED POLYTHIOPHENE; HYBRIDIZATION DETECTION; POLYANILINE NANOTUBES; POLY(O-ANISIDINE); COPOLYMERS; RESOLUTION;

D O I：

10.1016/j.eurpolymj.2012.09.009

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Poly(o-methoxyaniline) (POMA) hollow nanospheres diameter in 210 nm were prepared in a solution of poly(methyl vinyl etheralt-maleic acid) (PMVEA) by a self-assembly process using ammonium persulfate CAPS) as the oxidant. The morphology and the amount of nanospheres of POMA in the final products are highly affected by the molar ratio of APS to o-methoxyaniline (OMA) and the concentration of OMA. The structure and property of POMA hollow nanospheres were characterized by FTIR and cyclic voltammetry. We constructed a label-free oligonucleotide (ODN) sensor using PMVEA-doped POMA nanospheres: PMVEA provides a high density of carboxylic acid groups for covalent grafting of the ODN probe. The hybridization of the complementary ODNs can be clearly detected by the decrease in the impedance without the need for an indicator or any sample modifications. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：139 / 146

页数：8

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