Label-Free Microscale Thermophoresis Discriminates Sites and Affinity of Protein-Ligand Binding

被引：138

作者：

Seidel, Susanne A. I. ^{[2
]}

Wienken, Christoph J. ^{[2
]}

Geissler, Sandra ^{[1
]}

Jerabek-Willemsen, Moran ^{[1
]}

Duhr, Stefan ^{[1
]}

Reiter, Alwin ^{[3
,4
]}

Trauner, Dirk ^{[3
,4
]}

Braun, Dieter ^{[2
]}

Baaske, Philipp ^{[1
]}

机构：

[1] NanoTemper Technol GmbH, D-81369 Munich, Germany

[2] Univ Munich, D-80799 Munich, Germany

[3] Univ Munich, Dept Chem & Biochem, D-81377 Munich, Germany

[4] Univ Munich, Ctr Integrated Prot Sci, D-81377 Munich, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 42期

关键词：

analytical methods; binding affinity; label-free methods; microscale thermophoresis; protein conformation;

D O I：

10.1002/anie.201204268

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

[No abstract available]

引用

页码：10656 / 10659

页数：4

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