How has dental pulp stem cells isolation been conducted? A scoping review

被引：16
作者：
Ferrua, Camila Perello [1 ]
Zampieri Centeno, Eduarda Gervini [2 ]
da Rosa, Laisa Camerini [1 ]
do Amaral, Caina Correa [1 ]
Severo, Rafaely Ferreira [1 ]
Sarkis-Onofre, Rafael [3 ]
Nascimento, Gustavo Giacomelli [4 ]
Cordenonzi, Gabriele [1 ]
Bast, Rachel Krolow [5 ]
Demarco, Flavio Fernando [4 ]
Nedel, Fernanda [1 ]
机构：
[1] Univ Catolica Pelotas UCPel, Program Hlth & Behav, Pelotas, RS, Brazil
[2] Univ Fed Pelotas UFPel, Grad Program Biotecnol, Technol Dev Ctr, Pelotas, RS, Brazil
[3] IMED, Grad Program Dent, Passo Fundo, RS, Brazil
[4] Univ Fed Pelotas UFPel, Grad Program Dent, Pelotas, RS, Brazil
[5] Univ Fed Rio Grande do Sul, Inst Basic Hlth Scieces, Biochem Dept, Porto Alegre, RS, Brazil
来源：
BRAZILIAN ORAL RESEARCH | 2017年 / 31卷
关键词：
Dental Pulp; Stem Cells; Review; GLYCOLYLNEURAMINIC ACID XENOANTIGEN; IN-VITRO; CYTOKINE RELEASE; DIFFERENTIATION; COLLAGEN; ENHANCE; NEURONS; BLOOD;
D O I：
10.1590/1807-3107BOR-2017.vol31.0087
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
The objective of this study was to realize a scoping review the literature in order to identify the profile of DPSCs isolation and analyze the possible risk factors that could change the native behavior of these cells. An initial search was conducted using the following MeSH terms: "(dental pulp stem cell [MeSH])"; "(dental pulp [MeSH])" AND "(stem cell [MeSH])"; "("dental pulp stem cell" [MeSH]")". The electronic search was done without date restriction up to and including April 2014, in PubMed, Scopus, Scielo and ISI Web of Knowledge databases. Studies were submitted to inclusion and exclusion criteria and 222 articles were included. Data showed that over the past 15 years many studies have been conducted using DPSCs. However this is the first systematic review regarding the isolation of stem cell, and more specifically of dental pulp stem cells. The isolation of dental pulp stem cells showed great variability, hampering the development of standard protocols to achieve in vitro dental pulp stem cells with similar characteristics. This scoping review combined, for the first time, the methodologies used for dental pulp stem isolation, highlighting the most frequently used.
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页数：9
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