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Biofilms as a sink for antibiotic resistance genes (ARGs) in the Yangtze Estuary
被引:230
作者:
Guo, Xing-pan
[1
]
Yang, Yi
[1
,2
]
Lu, Da-pei
[1
]
Niu, Zuo-shun
[1
]
Feng, Jing-nan
[1
]
Chen, Yu-ru
[1
]
Tou, Fei-yun
[1
]
Garner, Emily
[3
]
Xu, Jiang
[4
]
Liu, Min
[1
]
Hochella, Michael F., Jr.
[5
,6
]
机构:
[1] East China Normal Univ, Sch Geog Sci, Minist Educ, Key Lab Geog Informat Sci, 500 Dongchuan Rd, Shanghai 200241, Peoples R China
[2] East China Normal Univ, State Key Lab Estuarine & Coastal Res, 3663 North Zhongshan Rd, Shanghai 200062, Peoples R China
[3] Virginia Tech, Charles E Via Jr Dept Civil & Environm Engn, 418 Durham Hall, Blacksburg, VA 24061 USA
[4] Carnegie Mellon Univ, Dept Civil & Environm Engn, Pittsburgh, PA 15213 USA
[5] Virginia Tech, Ctr NanoBioEarth, Dept Geosci, Blacksburg, VA 24061 USA
[6] Pacific Northwest Natl Lab, Geosci Grp, Energy & Environm Directorate, Richland, WA 99352 USA
来源:
基金:
中国国家自然科学基金;
关键词:
Biofilm;
Antibiotic resistance genes;
Yangtze estuary;
Extracellular DNA;
Partitioning coefficients;
WASTE-WATER;
TETRACYCLINE RESISTANCE;
SURFACE SEDIMENTS;
LARVAL SETTLEMENT;
METAL RESISTANCE;
AQUATIC SYSTEMS;
COASTAL AREAS;
RIVER ESTUARY;
HAIHE RIVER;
CHINA;
D O I:
10.1016/j.watres.2017.11.029
中图分类号:
X [环境科学、安全科学];
学科分类号:
08 ;
0830 ;
摘要:
Bioilms are ubiquitous throughout aquatic environments and they are thought to promote the acquisition and dissemination of antibiotic resistant genes (ARGs). This study focused on the occurrence and distribution of five types of ARG in naturally-occurring biofilms, in comparison to associated sediment and water samples, from the Yangtze Estuary, which borders the meta-city of Shanghai, China. The detection frequency and abundances of most ARGs showed the following order: biofilm > sediment > water, which can be attributed to a high level of antibiotics and metals that can accelerate the generation and propagation of ARGs in biofilms. Most of ARG abundances were contributed by extracellular DNA (eDNA) in biofilm and sediment samples. ARGs (sul1, sul2, tetA and tetW) in eDNA were significantly correlated with TOC in both biofilm and sediment samples. Furthermore, both intracellular DNA-associated ARGs per gram of microbial biomass carbon (MBC) and eDNA-associated ARGs per gram of non-MBC and were higher in biofilms than sediments, and the partitioning coefficients of ARGs in eDNA between biofilm and water were higher than those between sediment and water. Our results provide new insight for evaluating the occurrence and abundance of ARGs in aquatic environments, confirming that biofilms are a significant sink for ARGs in the estuarine environment. (C) 2017 Elsevier Ltd. All rights reserved.
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页码:277 / 286
页数:10
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