Laboratory-scale purification of microcystins using flash chromatography and reversed-phase high-performance liquid chromatography

被引：39
作者：
Edwards, C
Lawton, LA
Coyle, SM
Ross, P
机构：
[1] ROBERT GORDON UNIV,SCH APPL SCI,ABERDEEN AB1 1HG,SCOTLAND
[2] SHANDON HPLC,RUNCORN WA7 1PR,CHESHIRE,ENGLAND
来源：
JOURNAL OF CHROMATOGRAPHY A | 1996年 / 734卷 / 01期
关键词：
flash chromatography; preparative chromatography; peptides; microcystins;
D O I：
10.1016/0021-9673(95)01004-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Microcystins were extracted from 71 (equivalent to 313 g dry weight) of cyanobacterial scum collected from Rutland Water in Leicestershire, UK in 1989. The resulting aqueous extract was rapidly concentrated on a C-18 flash chromatography cartridge and microcystins were eluted using a step gradient. Fractions were collected manually and monitored by UV spectrophotometer and analytical HPLC. Fractions containing microcystins of similar polarity were pooled to give three fractions. Simple isocratic methods for separating each fraction were developed on an analytical column and scaled up to a 15X7.5 cm I.D. column. Closed-loop recycling was used to maximise yield and purity of two hydrophobic microcystins.
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页码：163 / 173
页数：11
相关论文
共 15 条
[1]  
[Anonymous], 1994, PRIRODA
[2]   ISOLATION AND CHARACTERIZATION OF 4 TOXINS FROM THE BLUE-GREEN-ALGA, MICROCYSTIS-AERUGINOSA [J].
BOTES, DP ;
KRUGER, H ;
VILJOEN, CC .
TOXICON, 1982, 20 (06) :945-954
[3]   NAMING OF CYCLIC HEPTAPEPTIDE TOXINS OF CYANOBACTERIA (BLUE-GREEN-ALGAE) [J].
CARMICHAEL, WW ;
BEASLEY, V ;
BUNNER, DL ;
ELOFF, JN ;
FALCONER, I ;
GORHAM, P ;
HARADA, KI ;
KRISHNAMURTHY, T ;
YU, MJ ;
MOORE, RE ;
RINEHART, K ;
RUNNEGAR, M ;
SKULBERG, OM ;
WATANABE, M .
TOXICON, 1988, 26 (11) :971-973
[4]   THE STRUCTURE AND REGULATION OF PROTEIN PHOSPHATASES [J].
COHEN, P .
ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :453-508
[5]  
EDWARDS C, 1994, LAB EQUIP DIGEST DEC, P19
[6]   EVIDENCE OF LIVER-DAMAGE BY TOXIN FROM A BLOOM OF THE BLUE-GREEN-ALGA, MICROCYSTIS-AERUGINOSA [J].
FALCONER, IR ;
BERESFORD, AM ;
RUNNEGAR, MTC .
MEDICAL JOURNAL OF AUSTRALIA, 1983, 1 (11) :511-514
[7]  
GORHAM PR, 1988, ALGAE HUMAN AFFAIRS, P404
[8]   MICROCYSTINS FROM ANABAENA-FLOS-AQUAE NRC-525-17 [J].
HARADA, K ;
OGAWA, K ;
KIMURA, Y ;
MURATA, H ;
SUZUKI, M ;
THORN, PM ;
EVANS, WR ;
CARMICHAEL, WW .
CHEMICAL RESEARCH IN TOXICOLOGY, 1991, 4 (05) :535-540
[9]  
HINDLEY S, 1994, 20 INT S CHROM BOURN
[10]   EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF MICROCYSTINS IN RAW AND TREATED WATERS [J].
LAWTON, LA ;
EDWARDS, C ;
CODD, GA .
ANALYST, 1994, 119 (07) :1525-1530
12