Generation, characterization and therapeutic potential of anti-feline TNF-alpha MAbs for feline infectious peritonitis

被引：7
作者：
Doki, Tomoyoshi [1 ]
Takano, Tomomi [1 ]
Nishiyama, Yuri [1 ]
Nakamura, Michiyo [1 ]
Hohdatsu, Tsutomu [1 ]
机构：
[1] Kitasato Univ, Sch Vet Med, Lab Vet Infect Dis, Towada, Aomori 0348628, Japan
来源：
RESEARCH IN VETERINARY SCIENCE | 2013年 / 95卷 / 03期
关键词：
Feline coronavirus; Feline infectious peritonitis; Tumor necrosis factor-alpha; TUMOR-NECROSIS-FACTOR; MONOCLONAL-ANTIBODY; CORONAVIRUS; MACROPHAGES; ENHANCEMENT; LYMPHOCYTES; INDUCTION; CATS;
D O I：
10.1016/j.rvsc.2013.09.005
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Feline infectious peritonitis (FIP) is a lethal infectious disease affecting domestic and wild cats. Several reports suggested that TNF-alpha is related to the progression of FIP. Thus, the administration of a feline TNF-alpha-neutralizing antibody to cats with FIP may reduce the disease progression. In this study, we have prepared nine monoclonal antibodies (MAbs) that recognize feline TNF-alpha. All MAbs neutralized recombinant TNF-alpha. The 50% inhibitory concentrations (IC50) of the MAbs for the cytotoxicity of recombinant TNF-alpha were 5-684 ng/ml. MAb 2-4 exhibited high neutralizing activity against natural TNF-alpha derived from FIPV-infected macrophages, and was confirmed to inhibit the following feline TNF-alpha-induced conditions in vitro: (i) an increase in the survival rate of neutrophils from cats with FIP, (ii) aminopeptidase N (APN) mRNA expression in macrophages, and (iii) apoptosis of a feline T-lymphocyte cell line. (C) 2013 Elsevier Ltd. All rights reserved.
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收藏
页码：1248 / 1254
页数：7
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