Quantitative determination of Closantel residues in milk by high-performance liquid chromatography with fluorescence detection

被引：15

作者：

Stoev, G ^{[1
]}

Dakova, T ^{[1
]}

Michailova, A ^{[1
]}

机构：

[1] Cent Lab Vet Control & Ecol, Sofia 1528, Bulgaria

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 846卷 / 1-2期

关键词：

milk; food analysis; extraction methods; Closantel;

D O I：

10.1016/S0021-9673(99)00135-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A HPLC method with fluorescence detection for quantitative determination of Closantel residues in milk has been developed and validated. The proposed cleaning procedure with acetonitrile and acetone extraction, and solid-phase clean-up with Florisil enables concentrations of Closantel below 50 mu g/l to be determined. The method was shown to be sufficient, precise, accurate, selective and rugged. The method was applied in the regular monitoring of Closantel residues in milk and of the pharmacokinetic behavior of Closantel in sheep. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：383 / 386

页数：4

共 7 条

[1] DETERMINATION OF RAFOXANIDE AND CLOSANTEL IN OVINE PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].