Effects of Captopril on Melanin Formation in B16 cells

被引：4

作者：

Chu, Heuy-Ling ^{[1
]}

Wang, Bor-Sen ^{[2
]}

Chang, Li-Chen ^{[3
]}

Chang, Lee-Wen ^{[1
]}

Duh, Pin-Der ^{[1
]}

机构：

[1] Chia Nan Univ Pharm & Sci, Dept Food Sci & Technol, Tainan, Taiwan

[2] Chia Nan Univ Pharm & Sci, Dept Appl Life Sci & Healty, Tainan, Taiwan

[3] Chia Nan Univ Pharm & Sci, Dept Pharm, Tainan, Taiwan

来源：

JOURNAL OF FOOD AND DRUG ANALYSIS | 2012年 / 20卷 / 03期

关键词：

captopril; antityrosinase; reactive oxygen species; melanin formation; B16 melanoma cell; TYROSINASE INHIBITORY-ACTIVITY; POLYPHENOL OXIDASE; IN-VITRO; KAPPA-B; ANTIOXIDANT; PIGMENTATION; INDUCTION; MECHANISM; OXIDATION; PROTEINS;

D O I：

10.6227/jfda.2012200314

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The effect of captopril on melanin formation in B16 cells was investigated. In B16 intracellular model system, the effect of captopril on antityrosinase activity was found dose-dependent and correlated to its ability to suppress melanin formation. The copper chelation by captopril may in part be responsible for the inhibition of tyrosinase activity. Captopril also displayed a remarkable reducing ability and significantly inhibited ROS generation. Interestingly, captopril also significantly suppressed tyrosinase mRNA expression, as determined by reverse transcription-polymerase chain reaction (RT-PCR). Overall, the results showed that the protective effect of captopril makes it a potent inhibitor of melanin formation.

引用

页码：668 / 673

页数：6

共 30 条

[1] N-acetylcysteine selectively protects cerebellar granule cells from 4-hydroxynonenal-induced cell death [J].

Arakawa, Motoki ;

Ushimaru, Nobuyuki ;

Osada, Nobuhiro ;

Oda, Tetsuro ;

Ishige, Kumiko ;

Ito, Yoshihisa .

NEUROSCIENCE RESEARCH, 2006, 55 (03) :255-263

[2] Lipoxygenase/H2O2-catalyzed oxidation of dihydroxyindoles:: Synthesis of melanin pigments and study of their antioxidant properties [J].

Blarzino, C ;

Mosca, L ;

Foppoli, C ;

Coccia, R ;

De Marco, C ;

Rosei, MA .

FREE RADICAL BIOLOGY AND MEDICINE, 1999, 26 (3-4) :446-453

[3] Use of experimental design methodology to prepare Maillard reaction products from glucose and cysteine inhibitors of polyphenol oxidase from eggplant (Solanum melongena) [J].

Cheriot, Sophie C. ;

Billaud, Catherine ;

Nicolas, Jacques .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (14) :5120-5126

[4] Antioxidant and antimelanogenic activities of polyamine conjugates from corn bran and related hydroxycinnamic acids [J].

Choi, Sang Won ;

Lee, Sung Kwon ;

Kim, Eun Ok ;

Oh, Ji Hae ;

Yoon, Kyung Sup ;

Parris, Nicholas ;

Hicks, Kevin B. ;

Moreau, Robert A. .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (10) :3920-3925

[5] Effects of Selected Organo-sulfur Compounds on Melanin Formation [J].

Chu, Heuy-Ling ;

Wang, Bor-Sen ;

Duh, Pin-Der .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (15) :7072-7077

[6] Tyrosinase and related proteins in mammalian pigmentation [J].

delMarmol, V ;

Beermann, F .

FEBS LETTERS, 1996, 381 (03) :165-168

[7] Biologically important Thiols in various vegetables and fruits [J].

Demirkol, O ;

Adams, C ;

Ercal, N .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (26) :8151-8154

[8] Effects of hydrogen peroxide treatment on thiol contents in fresh-cut asparagus (Asparagus officinalis) spears [J].

Demirkol, Omca .

INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, 2009, 60 (01) :80-88

[9]

Duh PD, 1999, FOOD SCI TECHNOL-LEB, V32, P269

[10] Effect of captopril on mushroom tyrosinase activity in vitro [J].

Espín, JC ;

Wichers, HJ .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2001, 1544 (1-2) :289-300

← 1 2 3 →