Pulse front adaptive optics in multiphoton microscopy

被引：0
作者：
Sun, B. [1 ,2 ]
Salter, P. S. [1 ]
Booth, M. J. [1 ,2 ,3 ]
机构：
[1] Univ Oxford, Dept Engn Sci, Parks Rd, Oxford OX1 3PJ, England
[2] Friedrich Alexander Univ Erlangen Nurnberg, Sch Adv Opt Technol, Erlangen, Germany
[3] Univ Oxford, Ctr Neural Circuits & Behav, Oxford OX1 2JD, England
来源：
ADAPTIVE OPTICS AND WAVEFRONT CONTROL FOR BIOLOGICAL SYSTEMS II | 2016年 / 9717卷
关键词：
Adaptive optics; Multiphoton microscopy; Ultrafast optics; Spatial light modulators; Deformable mirrors; FEMTOSECOND LASER-PULSES; DISTORTION; ABERRATIONS; LENSES;
D O I：
10.1117/12.2212642
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
The accurate focusing of ultrashort laser pulses is extremely important in multiphoton microscopy. Using adaptive optics to manipulate the incident ultrafast beam in either the spectral or spatial domain can introduce significant benefits when imaging. Here we introduce pulse front adaptive optics: manipulating an ultrashort pulse in both the spatial and temporal domains. A deformable mirror and a spatial light modulator are operated in concert to modify contours of constant intensity in space and time within an ultrashort pulse. Through adaptive control of the pulse front, we demonstrate an enhancement in the measured fluorescence from a two photon microscope.
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页数：4
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