Regional expression of the BCRP/ABCG2 transporter in term human placentas

被引：14
作者：
Memon, Naureen [1 ]
Bircsak, Kristin M. [2 ]
Archer, Faith [1 ]
Gibson, Christopher J. [2 ]
Ohman-Strickland, Pamela [3 ]
Weinberger, Barry I. [1 ]
Parast, Mana M. [4 ]
Vetrano, Anna M. [1 ]
Aleksunes, Lauren M. [2 ,5 ]
机构：
[1] Rutgers State Univ, Robert Wood Johnson Med Sch, Dept Pediat, New Brunswick, NJ 08901 USA
[2] Rutgers State Univ, Dept Pharmacol & Toxicol, Ernest Mario Sch Pharm, Piscataway, NJ 08854 USA
[3] Rutgers State Univ, Dept Biostat, Sch Publ Hlth, Piscataway, NJ 08854 USA
[4] Univ Calif San Diego, Dept Pathol, San Diego, CA 92035 USA
[5] Environm & Occupat Hlth Sci Inst, Piscataway, NJ 08854 USA
来源：
REPRODUCTIVE TOXICOLOGY | 2014年 / 43卷
基金：
美国国家卫生研究院;
关键词：
BCRP; ABCG2; Transporter; Placenta; Housekeeping gene; CANCER RESISTANCE PROTEIN; LIMITS FETAL DISTRIBUTION; GENE-EXPRESSION; GROWTH RESTRICTION; MULTIDRUG-RESISTANCE; PREGNANT MOUSE; SAMPLING SITE; GLYBURIDE; SURVIVAL; TISSUES;
D O I：
10.1016/j.reprotox.2013.11.003
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The breast cancer resistance protein (BCRP, ABCG2) is an efflux transporter that removes xenobiotics that cross the placenta back to the maternal circulation, thereby limiting exposure of the fetus to drugs and chemicals. Currently, variability of BCRP expression within the placenta is not known. Ten placentas were collected from healthy women undergoing elective Cesarean sections at term. Villous samples were dissected in defined regions (medial, intermediate, and peripheral) and BCRP mRNA and protein were quantified. There were no regional differences in mRNA expression of housekeeping genes (GAPDH, RPL13a, PRL, 18S). GAPDH had the lowest correlation with BCRP Ct values and was used for BCRP mRNA normalization. No differences in placental BCRP mRNA and protein were observed among the sample sites (<20% variability). Sampling site does not affect the expression of BCRP, supporting the utility of single site sampling protocols to assess the interindividual regulation of this transporter in human placentas. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码：72 / 77
页数：6
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