Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury

被引：31

作者：

Costa Rodrigues Pinheiro, Aruana Joaquim Matheus ^{[1
,2
,3
]}

Goncalves, Jaciara Sa ^{[1
]}

Alves Dourado, Adylla Wilenna ^{[1
]}

de Sousa, Eduardo Martins ^{[1
]}

Brito, Natilene Mesquita ^{[4
]}

Silva, Lanna Karinny ^{[4
]}

Aranha Batista, Marisa Cristina ^{[5
]}

de Sa, Joicy Cortez ^{[6
]}

Aragao Vieira Monteiro, Cinara Regina ^{[1
,7
]}

Fernandes, Elizabeth Soares ^{[1
]}

Monteiro-Neto, Valerio ^{[1
,7
]}

Campbell, Lee Ann ^{[8
]}

Wiziack Zago, Patricia Maria ^{[9
]}

Lima-Neto, Lidio Goncalves ^{[1
,2
]}

机构：

[1] Univ CEUMA, Programa Posgrad, Sao Luis, MA, Brazil

[2] Univ Fed Amazonas, Programa Posgrad Rede BIONORTE, Manaus, AM, Brazil

[3] Fac Pitagoras, Dept Farm, Sao Luis, MA, Brazil

[4] Inst Fed Maranhao, Lab Estudos Ambientais, Sao Luis, MA, Brazil

[5] Univ Fed Maranhao, Lab Farmacognosia 1, Sao Luis, MA, Brazil

[6] Univ CEUMA, Dep Medicina, Sao Luis, MA, Brazil

[7] Univ Fed Maranhao, Ctr Ciencias Biol & Saude, Sao Luis, MA, Brazil

[8] Univ Washington, Dept Global Hlth, Seattle, WA 98195 USA

[9] Univ CEUMA, Dept Odontol, Sao Luis, MA, Brazil

来源：

JOURNAL OF IMMUNOLOGY RESEARCH | 2018年 / 2018卷

关键词：

POMEGRANATE; EXPRESSION;

D O I：

10.1155/2018/6879183

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The hydroalcoholic extract of Punica granatum (pomegranate) leaves was previously demonstrated to be anti-inflammatory in a rat model of lipopolysaccharide- (LPS-) induced acute peritonitis. Here, we investigated the anti-inflammatory effects of the ethyl acetate fraction obtained from the pomegranate leaf hydroalcoholic extract (EAFPg) on the LPS-induced acute lung injury (ALI) mouse model. Male Swiss mice received either EAFPg at different doses or dexamethasone (per os) prior to LPS intranasal instillation. Vehicle-treated mice were used as controls. Animals were culled at 4 h after LPS challenge, and the bronchoalveolar lavage fluid (BALF) and lung samples were collected for analysis. EAFPg and kaempferol effects on NO and cytokine production by LPS-stimulated RAW 264.7 macrophages were also investigated. Pretreatment with EAFPg (100-300 mg/kg) markedly reduced cell accumulation (specially neutrophils) and collagen deposition in the lungs of ALI mice. The same animals presented with reduced lung and BALF TNF-alpha and IL-1 beta expression in comparison with vehicle controls (p < 0 05). Additionally, incubation with either EAFPg or kaempferol (100 mu g/ml) reduced NO production and cytokine gene expression in cultured LPS-treated RAW 264.7 macrophages. Overall, these results demonstrate that the prophylactic treatment with EAFPg attenuates acute lung inflammation. We suggest this fraction may be useful in treating ALI.

引用

页数：11

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