Aims: The root of Polygonatum odoratum (YuZhu), also a medicinal food has long been used for the treatment of diabetes. The objective of the study was to characterize the anti-diabetic active fractions or compounds in this herb. Materials and methods: Fractions with a different polarity were prepared by solvent extraction and macroporous absorptive resin (D101) column and their anti-diabetic potentials were evaluated by glucose uptake in HepG2 cells and STZ-induced diabetic rats. In addition, alpha-glycosidase inhibitory activities of active fractions were measured in vitro and chemical compositions including saponin, total flavonoids and total sugar in the fractions were determined. Results: The n-buthanol fraction, a saponin-rich fraction obtained by partitioning the ethanol extract with n-buthanol after petroleum ether and acetic ether showed the highest anti-diabetic potential in glucose uptake in HepG2 cells followed by acetic ether fraction which was rich in flavonoids. Further fractionation the saponin-rich fraction using macroporous resin column (D101), polysaccharide, flavonoid and saponin rich fractions were obtained by elution with water, 40% and 60% ethanol, respectively and their antidiabetic potentials proved by glucose uptake test in HepG2 cells and STZ-induced diabetic rats were in the order of saponin rich fraction > flavonoid rich fraction > polysaccharide rich fraction. Long-term therapy test (60 d) in severe diabetic rats indicated that saponin-rich fraction significantly ameliorated clinical symptoms of diabetes including the elevated blood glucose, body weight loss as well as the increased food and water intake while flavonoid-rich fraction was more potential than saponin-rich fraction to increase superoxide dismutase (SOD) activity and decrease malondialdehyde (MDA) level in rat plasma. Additionally, saponin-rich fraction and flavonoid-rich fraction showed alpha-glycosidase inhibitory activity with IC50 value of 2.05 +/- 0.32 and 3.92 +/- 0.65 mg/ml, respectively. Conclusion: The results suggested that saponin in this herb was more important than flavonoid in exhibiting anti-diabetic activity and flavonoid contributed more to anti-oxidant activity in vivo. (c) 2012 Elsevier Ireland Ltd. All rights reserved.
机构:Univ Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, India
Gupta, RK
Kesari, AN
论文数: 0引用数: 0
h-index: 0
机构:Univ Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, India
Kesari, AN
Murthy, PS
论文数: 0引用数: 0
h-index: 0
机构:Univ Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, India
Murthy, PS
Chandra, R
论文数: 0引用数: 0
h-index: 0
机构:Univ Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, India
Chandra, R
Tandon, V
论文数: 0引用数: 0
h-index: 0
机构:Univ Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, India
Tandon, V
Watal, G
论文数: 0引用数: 0
h-index: 0
机构:
Univ Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, IndiaUniv Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, India
机构:Univ Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, India
Gupta, RK
Kesari, AN
论文数: 0引用数: 0
h-index: 0
机构:Univ Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, India
Kesari, AN
Murthy, PS
论文数: 0引用数: 0
h-index: 0
机构:Univ Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, India
Murthy, PS
Chandra, R
论文数: 0引用数: 0
h-index: 0
机构:Univ Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, India
Chandra, R
Tandon, V
论文数: 0引用数: 0
h-index: 0
机构:Univ Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, India
Tandon, V
Watal, G
论文数: 0引用数: 0
h-index: 0
机构:
Univ Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, IndiaUniv Allahabad, Dept Chem, Drug Dev Div, Med Res Lab,Alternat Therapeut Unit, Allahabad 211002, Uttar Pradesh, India