Saponin rich fractions from Polygonatum odoratum (Mill.) Druce with more potential hypoglycemic effects

被引:75
作者
Deng, Yafei [1 ]
He, Kai [2 ]
Ye, Xiaoli [2 ]
Chen, Xin [1 ]
Huang, Jing [1 ]
Li, Xuegang [1 ]
Yuan, Lujiang [1 ]
Jin, Yalan [1 ]
Jin, Qing [1 ]
Li, Panpan [1 ]
机构
[1] Southwest Univ, Chem Inst Pharmaceut Resources, Coll Pharmaceut Sci, Chongqing 400716, Peoples R China
[2] Southwest Univ, Sch Life Sci, Chongqing 400715, Peoples R China
基金
国家科技攻关计划;
关键词
Saponin; Flavonoids; alpha-Glycosidase; Hypoglycemic; Polygonatum odoratum (Mill.) Druce; TYPE-2; DIABETIC-PATIENTS; OXIDATIVE STRESS; SULFURIC-ACID; IN-VITRO; RATS; EXTRACT; HYPERGLYCEMIA; THERAPY; MICE; ANTIOXIDANTS;
D O I
10.1016/j.jep.2012.02.023
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Aims: The root of Polygonatum odoratum (YuZhu), also a medicinal food has long been used for the treatment of diabetes. The objective of the study was to characterize the anti-diabetic active fractions or compounds in this herb. Materials and methods: Fractions with a different polarity were prepared by solvent extraction and macroporous absorptive resin (D101) column and their anti-diabetic potentials were evaluated by glucose uptake in HepG2 cells and STZ-induced diabetic rats. In addition, alpha-glycosidase inhibitory activities of active fractions were measured in vitro and chemical compositions including saponin, total flavonoids and total sugar in the fractions were determined. Results: The n-buthanol fraction, a saponin-rich fraction obtained by partitioning the ethanol extract with n-buthanol after petroleum ether and acetic ether showed the highest anti-diabetic potential in glucose uptake in HepG2 cells followed by acetic ether fraction which was rich in flavonoids. Further fractionation the saponin-rich fraction using macroporous resin column (D101), polysaccharide, flavonoid and saponin rich fractions were obtained by elution with water, 40% and 60% ethanol, respectively and their antidiabetic potentials proved by glucose uptake test in HepG2 cells and STZ-induced diabetic rats were in the order of saponin rich fraction > flavonoid rich fraction > polysaccharide rich fraction. Long-term therapy test (60 d) in severe diabetic rats indicated that saponin-rich fraction significantly ameliorated clinical symptoms of diabetes including the elevated blood glucose, body weight loss as well as the increased food and water intake while flavonoid-rich fraction was more potential than saponin-rich fraction to increase superoxide dismutase (SOD) activity and decrease malondialdehyde (MDA) level in rat plasma. Additionally, saponin-rich fraction and flavonoid-rich fraction showed alpha-glycosidase inhibitory activity with IC50 value of 2.05 +/- 0.32 and 3.92 +/- 0.65 mg/ml, respectively. Conclusion: The results suggested that saponin in this herb was more important than flavonoid in exhibiting anti-diabetic activity and flavonoid contributed more to anti-oxidant activity in vivo. (c) 2012 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:228 / 233
页数:6
相关论文
共 30 条
  • [1] STREPTOZOTOCIN DIABETES IN MOUSE AND GUINEA PIG
    BROSKY, G
    LOGOTHETOPOULOS, J
    [J]. DIABETES, 1969, 18 (09) : 606 - +
  • [2] Meal-generated oxidative stress in type 2 diabetic patients
    Ceriello, A
    Lizzio, S
    Bortolotti, N
    Russo, A
    Motz, E
    Tonutti, L
    Crescentini, A
    Taboga, C
    [J]. DIABETES CARE, 1998, 21 (09) : 1529 - 1533
  • [3] Chang CC, 2002, J FOOD DRUG ANAL, V10, P178
  • [4] Hypoglycemic effects of aqueous extract of Rhizoma Polygonati Odorati in mice and rats
    Chen, HB
    Feng, RZ
    Guo, Y
    Sun, LH
    Jiang, JL
    [J]. JOURNAL OF ETHNOPHARMACOLOGY, 2001, 74 (03) : 225 - 229
  • [5] China Pharmacopoeia Committee, 2010, PHARM PEOPL REP CHIN, P78
  • [6] A steroidal glycoside from Polygonatum odoratum (Mill.) Druce. improves insulin resistance but does not alter insulin secretion in 90% pancreatectomized rats
    Choi, SB
    Park, S
    [J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2002, 66 (10) : 2036 - 2043
  • [7] Hyperglycemia induced oxidative stress in type-1 and type-2 diabetic patients with and without nephropathy
    Dave, G. S.
    Kalia, K.
    [J]. CELLULAR AND MOLECULAR BIOLOGY, 2007, 53 (05) : 68 - 78
  • [8] The flavonoid-rich fraction of Coreopsis tinctoria promotes glucose tolerance regain through pancreatic function recovery in streptozotocin-induced glucose-intolerant rats
    Dias, Teresa
    Bronze, Maria Rosario
    Houghton, Peter J.
    Mota-Filipe, Helder
    Paulo, Alexandra
    [J]. JOURNAL OF ETHNOPHARMACOLOGY, 2010, 132 (02) : 483 - 490
  • [9] Hypoglycemic and antidiabetic effect of ethanolic extract of leaves of Annona squamosa L. in experimental animals
    Gupta, RK
    Kesari, AN
    Murthy, PS
    Chandra, R
    Tandon, V
    Watal, G
    [J]. JOURNAL OF ETHNOPHARMACOLOGY, 2005, 99 (01) : 75 - 81
  • [10] Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships
    Heim, KE
    Tagliaferro, AR
    Bobilya, DJ
    [J]. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2002, 13 (10) : 572 - 584