Cryptosporidium bollandi n. sp. (Apicomplexa: Cryptosporidiiae) from angelfish (Pterophyllum scalare) and Oscar fish (Astronotus ocellatus)

被引：27
作者：
Bolland, S. J. [1 ]
Zahedi, Alireza [1 ]
Oskam, Charlotte [1 ]
Murphy, Brian [2 ]
Ryan, Una [1 ]
机构：
[1] Murdoch Univ, Coll Sci Hlth Educ & Engn, Vector & Waterborne Pathogens Res Grp, Perth, WA, Australia
[2] Univ Calif Davis, Sch Vet Med, Dept Pathol Microbiol & Immunol, Davis, CA USA
来源：
EXPERIMENTAL PARASITOLOGY | 2020年 / 217卷
关键词：
Cryptosporidium bollandi n. sp; 18S; Actin; Fish; Phylogeny; MARINE FISH; IDENTIFICATION; INFECTION; PARASITES; GENOTYPES; LOCI; 18S;
D O I：
10.1016/j.exppara.2020.107956
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The species name Cryptosporidium bollandi n. sp. is proposed for Cryptosporidium piscine genotype 2 based on morphological, biological and molecular characterisation. Phylogenetic analyses of 188 rRNA (18S) sequences revealed that C. bollandi n. sp. was most closely related to piscine genotype 4 (5.1% genetic distance) and exhibited genetic distances of 10.0%, 12.2% and 25.2% from Cryptosporidium molnari, Cryptosporidium huwi and Cryptosporidium scophthtalmi, respectively. At the actin locus, C. bollandi n. sp. was again most closely related to piscine genotype 4 (6.8% genetic distance) and exhibited 15.5% (C. molnari), 18.4% (C. huwi), 22.9% (C. scophthalmi) and up to 27.5% genetic distance from other Cryptosporidium spp. (Cryptosporidium fells). Phylogenetic analysis of concatenated 18S and actin sequences showed that C. bollandi n. sp. exhibited 12.9% (C. molnari) to 21.1% (C. cans) genetic distance from all other Cryptosporidium spp. Genetic data as well as previous histological analysis clearly supports the validity of C. bollandi n. sp. as a separate species.
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