Mass spectrometry-based proteome profiling of extracellular vesicles and their roles in cancer biology

被引:107
作者
Bandu, Raju [1 ]
Oh, Jae Won [1 ]
Kim, Kwang Pyo [1 ,2 ]
机构
[1] Kyung Hee Univ, Global Ctr Pharmaceut Ingredient Mat, Inst Nat Sci, Dept Appl Chem, Yongin, South Korea
[2] Kyung Hee Univ, Kyung Hee Med Sci Res Inst, Dept Biomed Sci & Technol, Seoul, South Korea
关键词
REVEAL DIFFERENTIAL EXPRESSION; MEMBRANE-VESICLES; QUANTITATIVE PROTEOMICS; MYELOID CELLS; EMERGING ROLE; EXOSOMES; MICROVESICLES; TUMOR; PROTEINS; PROMOTE;
D O I
10.1038/s12276-019-0218-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Over the past three decades, extracellular vesicles (EVs) have arisen as important mediators of intercellular communication that are involved in the transmission of biological signals between cells to regulate various biological processes. EVs are largely responsible for intercellular communication through the delivery of bioactive molecules, such as proteins, messenger RNAs (mRNAs), microRNAs (miRNAs), DNAs, lipids, and metabolites. EVs released from cancer cells play a significant role in signal transduction between cancer cells and the surrounding cells, which contributes to the formation of tumors and metastasis in the tumor microenvironment. In addition, EVs released from cancer cells migrate to blood vessels and flow into various biological fluids, including blood and urine. EVs and EV-loaded functional cargoes, including proteins and miRNAs, found in these biological fluids are important biomarkers for cancer diagnosis. Therefore, EV proteomics greatly contributes to the understanding of carcinogenesis and tumor progression and is critical for the development of biomarkers for the early diagnosis of cancer. To explore the potential use of EVs as a gateway to understanding cancer biology and to develop cancer biomarkers, we discuss the mass spectrometric identification and characterization of EV proteins from different cancers. Information provided in this review may help in understanding recent progress regarding EV biology and the potential roles of EVs as new noninvasive biomarkers and therapeutic targets.
引用
收藏
页码:1 / 10
页数:10
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