A Hydroxyquinoline-Based Unnatural Amino Acid for the Design of Novel Artificial Metalloenzymes

被引：27

作者：

Drienovska, Ivana ^{[1
]}

Scheele, Remkes A. ^{[1
]}

Gutierrez de Souza, Cora ^{[1
]}

Roelfes, Gerard ^{[1
]}

机构：

[1] Univ Groningen, Stratingh Inst Chem, Nijenborgh 4, NL-9747 AG Groningen, Netherlands

来源：

CHEMBIOCHEM | 2020年 / 21卷 / 21期

基金：

欧洲研究理事会;

关键词：

biocatalysis; hybrid catalysts; metalloenzymes; noncanonical amino acids; protein design; GENETIC INCORPORATION; COMPUTATIONAL DESIGN; TERMINAL ALKYNES; PROTEIN; COMPLEXES; BINDING; DERIVATIVES; 8-HYDROXYQUINOLINE; METALLOPROTEIN; SITES;

D O I：

10.1002/cbic.202000306

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined the potential of the noncanonical amino acid (8-hydroxyquinolin-3-yl)alanine (HQAla) for the design of artificial metalloenzymes. HQAla, a versatile chelator of late transition metals, was introduced into the lactococcal multidrug-resistance regulator (LmrR) by stop codon suppression methodology. LmrR_HQAla was shown to complex efficiently with three different metal ions, Cu-II, Zn(II)and Rh(III)to form unique artificial metalloenzymes. The catalytic potential of the Cu-II-bound LmrR_HQAla enzyme was shown through its ability to catalyse asymmetric Friedel-Craft alkylation and water addition, whereas the Zn-II-coupled enzyme was shown to mimic natural Zn hydrolase activity.

引用

页码：3077 / 3081

页数：5

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