Purification, chemical characterization and antioxidant activities of polysaccharides of fruiting bodies of Pleurotus ferulae mushroom

被引：6
作者：
Chen, Yiyong [1 ]
Gao, Lin [1 ]
Gao, Rong [1 ]
机构：
[1] Changshu Inst Technol, Sch Biol & Food Engn, Changshu 215500, Jiangsu, Peoples R China
来源：
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION | 2019年 / 13卷 / 01期
关键词：
Pleurotus ferulae; Polysaccharide; Antioxidant; Characterization; IN-VITRO; EXTRACTS;
D O I：
10.1007/s11694-018-9935-9
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Three water-soluble polysaccharides fractions (PFP1a, PFP2b and PFP3c) were isolated and purified from Pleurotus ferulae by DEAE-Sepharose CL-6B and Sepharose CL-6B column chromatography. Their chemical characterization was conducted by chemical methods, chromatography and Fourier transform infrared spectroscopy. Antioxidant activities of crude polysaccharides from P. ferulae (CPFP) and its purified fractions were investigated on the basis of superoxide anion, DPPH, hydroxyl radical scavenging activity and reducing power. The result indicated PFP1a, PFP2b and PFP3c had obvious characteristic peaks of polysaccharides and were mainly composed of six kinds of monosaccharides including rhamnose, arabinose, glucose, mannose, xylose and galactose. The average molecular weight of PFP1a, PFP2b and PFP3c were approximately 5.9x10(5)Da, 3.2x10(5)Da and 2.6x10(4)Da, respectively. PFP1a, PFP2b and PFP3c exhibited antioxidant activities in a concentration-dependent manner. The results demonstrated that the three polysaccharides had the potential to be new natural antioxidant in the food and pharmaceutical industry, especially the PFP2b and PFP3c containing more uronic acid.
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页码：222 / 230
页数：9
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