Antiangiogenic effects of catalpol on rat corneal neovascularization

被引：19

作者：

Han, Yun ^{[1
]}

Shen, Mei ^{[1
]}

Tang, Li-Yuan ^{[2
]}

Tan, Gang ^{[3
]}

Yang, Qi-Chen ^{[1
]}

Ye, Lei ^{[2
]}

Ye, Lin-Hong ^{[2
]}

Jiang, Nan ^{[2
]}

Gao, Gui-Ping ^{[2
]}

Shao, Yi ^{[2
]}

机构：

[1] Xiamen Univ, Med Coll, Fujian Prov Key Lab Ophthalmol & Visual Sci, Eye Inst, Xiamen 361102, Fujian, Peoples R China

[2] Nanchang Univ, Dept Ophthalmol, Affiliated Hosp 1, 17 Yongwaizheng St, Nanchang 330006, Jiangxi, Peoples R China

[3] Univ South China, Dept Ophthalmol, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2018年 / 17卷 / 02期

基金：

中国国家自然科学基金;

关键词：

catalpol; corneal alkali burn; neovascularization; ENDOTHELIAL GROWTH-FACTOR; EPITHELIUM-DERIVED FACTOR; ALKALI BURNS; ANGIOGENESIS; CELLS; INHIBITOR; NETRIN-1; MICE;

D O I：

10.3892/mmr.2017.8114

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To investigate the effects of catalpol on corneal neovascularization (CNV) and associated inflammation, eye drops (5 mM catalpol or PBS) were administered four times daily to alkali-burn rat models of CNV and inflammation. Clinical evaluations of CNV and the degree of inflammation were performed on days 0, 4, 7, 10 and 14 under slit lamp microscopy. Eyes were collected on day 14 and prepared for hematoxylin and eosin, and immunofluorescence staining; corneal cell apoptosis was investigated via terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) staining. Protein expression levels of angiogenic and proinflammatory factors, including vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF), tumor necrosis factor-alpha (TNF-alpha) and necrosis factor-omicron kappa B (NF-kappa B) were determined by western blotting. The effects of catalpol on cell proliferation were investigated in vitro using human umbilical vein endothelial cells (HUVECs) and a Cell Counting kit-8 (CCK-8); alterations in migration and tube formation were investigated via HUVEC wound closure and tube formation assays. HUVEC viability and proliferative ability were inhibited in a dose-dependent manner; catalpol also decreased HUVEC cell migration and tube forming ability. Within alkali-burn rat models, decreased inflammation and CNV was associated with catalpol administration; as demonstrated with TUNEL, corneal cell apoptosis was decreased in response to catalpol. Western blot analysis revealed reduced protein expression levels of VEGF and TNF-alpha; however, PEDF and phosphorylated-NF-kappa B p65 were increased due to catalpol administration. The present study demonstrated the inhibitory effects exerted by catalpol on CNV and inflammation within alkali-burned rat models. Topical application of catalpol in vivo was associated with reduced CNV and inflammation; therefore, catalpol may be considered an anti-inflammatory agent for the clinical treatment of CNV.

引用

页码：2187 / 2194

页数：8

共 32 条

[1]

[Anonymous], 2001, Am J Public Health, V91, P476

[2] In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract [J].