Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells

被引：2
作者：
Akimoto, T
Uchida, T
Miyaki, S
Akaogi, H
Tateishi, T
Fukubayashi, T
机构：
[1] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Meguro Ku, Tokyo 1538902, Japan
[2] AIST, Tissue Engn Res Ctr, Tsukuba, Ibaraki 3058562, Japan
[3] Univ Tokyo, Grad Sch Engn, Biomed Engn Lab, Bunkyo Ku, Tokyo 1138656, Japan
来源：
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2004年 / 24卷 / 03期
关键词：
mechanical stretch; TGF-beta; 1; expression; C2C12 myogenic cells;
D O I：
10.1016/j.msec.2003.12.013
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-beta1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-beta1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-beta1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-beta1 expression on C2C12 myogenic satellite cell. (C) 2004 Published by Elsevier B.V.
引用
收藏
页码：387 / 389
页数：3
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