Porous silica microspheres obtained by grinding monolithic columns as stationary phase for high performance liquid chromatography

被引：4

作者：

Qu, Qishu ^{[1
]}

Gu, Qian ^{[1
]}

Shi, Lihua ^{[1
]}

Gu, Zuli ^{[1
]}

Hu, Xiaoya ^{[1
]}

机构：

[1] Yangzhou Univ, Coll Chem & Chem Engn, Jiangsu Key Lab Environm Mat & Environm Engn, Yangzhou 225002, Peoples R China

来源：

ANALYTICAL METHODS | 2012年 / 4卷 / 10期

关键词：

CAPILLARY COLUMNS; SIZE; SPHERES; SEPARATION; PARTICLES; MICRON; SBA-15;

D O I：

10.1039/c2ay25330e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new facile method of preparing porous spherical silica particles as stationary phase for high performance liquid chromatography (HPLC) separations was developed. In this method, monolithic silica columns whose skeletons contained weakly connected porous silica microspheres were prepared first using the aging temperature and mass ratio of PEG to TMOS in the range of 25-30 degrees C and 0.10-0.20, respectively, and mass ratio of TMOS to H2O less than 0.5. Then, dispersed porous silica spheres were obtained by gentle grinding of the monolithic column. After surface modification with C18, silica microspheres with an average diameter of 5.4 mu m were packed and used in HPLC for separation of neutral, acidic, and basic compounds. The retention behavior is similar to the column packed with commercially available 5 mu m C18 silica particles while column efficiency is only about a half of the latter. The results demonstrate that the microspheres are a promising alternative packing material for HPLC.

引用

页码：3200 / 3205

页数：6

共 30 条

[1] Relationship between the particle size distribution of commercial fully porous and superficially porous high-performance liquid chromatography column packings and their chromatographic performance [J].