Epidermal Growth Factor Attenuates Delayed Ionizing Radiation- Induced Tissue Damage in Bone Marrow Transplanted Mice

被引：1

作者：

Pejchal, Jaroslav ^{[1
]}

Sinkorova, Zuzana ^{[1
]}

Tichy, Ales ^{[1
,5
]}

Pruchova, Sarka ^{[1
]}

Kmochova, Adela ^{[1
]}

Durisova, Kamila ^{[1
]}

Cechakova, Lucie ^{[1
]}

Lierova, Anna ^{[1
]}

Ondrej, Martin ^{[1
]}

Nemcova, Marketa ^{[1
]}

Kubelkova, Klara ^{[2
]}

Fatorova, Ilona ^{[3
]}

Bures, Jan ^{[4
]}

Tacheci, Ilja ^{[4
]}

Kuca, Kamil ^{[5
]}

Vavrova, Jirina ^{[1
]}

机构：

[1] Univ Def, Fac Mil Hlth Sci, Dept Radiobiol, Trebesska 1575, Hradec Kralove 50001, Czech Republic

[2] Univ Def, Fac Mil Hlth Sci, Dept Mol Pathol & Biol, Hradec Kralove, Czech Republic

[3] Charles Univ Prague, Fac Med Hradec Kralove, Dept Internal Med 4, Haematol, Hradec Kralove, Czech Republic

[4] Charles Univ Prague, Fac Med Hradec Kralove, Dept Internal Med 2, Gastroenterol, Hradec Kralove, Czech Republic

[5] Univ Hosp, Biomed Reseach Ctr, Hradec Kralove, Czech Republic

来源：

RADIATION RESEARCH | 2016年 / 186卷 / 03期

关键词：

REGENERATION; EXPRESSION; CELLS;

D O I：

10.1667/RR14247.1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We examined the effect of epidermal growth factor (EGF) treatment in mice that received bone marrow transplantation (BMT) after 11 Gy whole-body irradiation. C57Bl/6 mice were divided into three treatment groups: 0 Gy; 11 Gy (Co-60, single dose, 0.51 Gy/min) with BMT (5 X 3 10(6) bone marrow cells isolated from green fluorescent protein syngeneic mice, 3-4 h postirradiation); and 11 Gy with BMT and EGF (2 mg/kg applied subcutaneously 1, 3 and 5 days postirradiation). Survival data were collected. Bone marrow, peripheral blood count and cytokines, gastrointestine and liver parameters and migration of green fluorescent protein-positive cells were evaluated at 63 days postirradiation. Epidermal growth factor increased survival of irradiated animals that received BMT from 10.7 to 85.7% at 180 days postirradiation. In the BMT group, we found changes in differential bone marrow and blood count, plasma cytokine levels, gastrointestinal tissues and liver at 63 days postirradiation. These alterations were completely or in some parameters at least partially restored by epidermal growth factor. These findings indicate that epidermal growth factor, administered 1, 3 and 5 days postirradiation in combination with bone marrow transplantation, significantly improves long-term prognosis. (c) 2016 by Radiation Research Society

引用

页码：264 / 274

页数：11