Effect of cultivation conditions on growth and α-amylase production by a thermophilic Bacillus sp.

被引：20

作者：

Mamo, G ^{[1
]}

Gessesse, A ^{[1
]}

机构：

[1] Univ Addis Ababa, Dept Biol, Addis Ababa, Ethiopia

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 29卷 / 01期

关键词：

D O I：

10.1046/j.1365-2672.1999.00577.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Growth and amylase production by Bacillus sp. WN11 was studied under different cultivation conditions. Maximum growth was observed at 65 degrees C while the highest amylase production was recorded at 55 degrees C. Good amylase production and growth was observed at an initial medium pH of 5 and 6. No detectable growth was observed at pH 4 and 9. The organism grew better and produced higher levels of amylase activity using proteose peptone and tryptone as nitrogen sources. No growth was observed when NO3+, NH4+ and urea were used as nitrogen sources. Among defined carbohydrates, tested starch and maltose supported good growth and amylase production, with the highest productivity recorded in the presence of starch (24 U/ml). Compared to starch, barley and corn flour resulted in maximum amylase production.

引用

页码：61 / 65

页数：5

共 31 条

[11] THE S-LAYER FROM BACILLUS-STEAROTHERMOPHILUS DSM-2358 FUNCTIONS AS AN ADHESION SITE FOR A HIGH-MOLECULAR-WEIGHT AMYLASE [J].